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  • Western Blot (WB) analysis of various lysates using YOD1 Recombinant antibody (84933-5-RR)

    WB analysis using 84933-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84933-5-RR (YOD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of K-562 cells using YOD1 Recombinant antibody (84933-5-RR)

    IF Staining of K-562 using 84933-5-RR

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using YOD1 antibody (84933-5-RR, Clone: 242498G6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84933-5-RR

    Affinity and Kinetic Characterization of 84933-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84933-5-RR against Human YOD1 were performed. The affinity constant is 1.07 nM.

YOD1 Recombinant antibody

YOD1 Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 84933-5-RR
Clone No.242498G6

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

242498G6, DUBA 8, DUBA8, DUBA-8, EC:3.4.19.12

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inK-562 cells, Jurkat cells, HEK-293 cells, HeLa cells
Positive IF/ICC detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84933-5-RR targets YOD1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen YOD1 fusion protein Ag21844 Predict reactive species
Full Name YOD1 OTU deubiquinating enzyme 1 homolog (S. cerevisiae)
Calculated Molecular Weight 348 aa, 38 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC137167
Gene Symbol YOD1
Gene ID (NCBI) 55432
Conjugate Unconjugated
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ5VVQ6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

YOD1, also known as YOD1 deubiquitinase, belongs to the ovarian tumor (OTU) domain-containing family of deubiquitinating enzymes (DUBs), which play a crucial role in regulating cellular processes by removing ubiquitin molecules from target proteins. YOD1 is particularly involved in the endoplasmic reticulum-associated degradation (ERAD) pathway, where it assists in the clearance of misfolded proteins from the endoplasmic reticulum (ER) by modulating the ubiquitination status of ERAD components. (PMID: 40132762,19818707)

Protocols

Product Specific Protocols
WB protocol for YOD1 antibody 84933-5-RRDownload protocol
IF protocol for YOD1 antibody 84933-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols