Tested Applications
| Positive WB detected in | ARPE-19 cells, Neuro-2a cells, Raji cells, SMMC-7721 cells, mouse testis tissue, rat testis tissue |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25629-1-AP targets ZCRB1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag22095 Product name: Recombinant human ZCRB1 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 1-217 aa of BC022543 Sequence: MSGGLAPSKSTVYVSNLPFSLTNNDLYRIFSKYGKVVKVTIMKDKDTRKSKGVAFILFLDKDSAQNCTRAINNKQLFGRVIKASIAIDNGRAAEFIRRRNYFDKSKCYECGESGHLSYACPKNMLGEREPQKKKEKKKKKKAPEPEEEIEEVEESEDEGEDPALDSLSQAIAFQQAKIEEEQKKWKPSSGVPSTSDDSRRPRIKKSTYFSDEEELSD Predict reactive species |
| Full Name | zinc finger CCHC-type and RNA binding motif 1 |
| Calculated Molecular Weight | 217 aa, 25 kDa |
| Observed Molecular Weight | 25-30 kDa |
| GenBank Accession Number | BC022543 |
| Gene Symbol | ZCRB1 |
| Gene ID (NCBI) | 85437 |
| RRID | AB_2880169 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8TBF4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ZCRB1 antibody 25629-1-AP | Download protocol |
| WB protocol for ZCRB1 antibody 25629-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ZCRB1 antibody (Cat# 25629-1-AP) has 5 total citations. Citations appear in J Exp Clin Cancer Res, Transl Oncol, Cell Signal, Life Sci Alliance, and bioRxiv. Research fields include glioblastoma metabolism, hepatocellular carcinoma progression, lung squamous cell carcinoma proliferation and metastasis, ciliogenesis, and WNT signaling pathways.
| Species | Application | Title |
|---|---|---|
J Exp Clin Cancer Res A novel protein encoded by ZCRB1-induced circHEATR5B suppresses aerobic glycolysis of GBM through phosphorylation of JMJD5. | ||
Transl Oncol 3'UTR shortening of ZCRB1 promotes malignant progression of hepatocellular carcinoma through GOLM1. | ||
Cell Signal ZCRB1 promotes proliferation and metastasis of lung squamous cell carcinoma by stabilizing DSG3 mRNA. | ||
Life Sci Alliance Loss of U11/U12 spliceosome gene ZCRB1 leads to aberrant ciliogenesis and WNT signaling.
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