Tested Applications
| Positive WB detected in | mouse brain tissue, SK-N-SH cells, rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
82739-1-RR targets ZDHHC9 in WB, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag20881 Product name: Recombinant human ZDHHC9 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 87-364 aa of BC006200 Sequence: SFSDPGVIPRALPDEAAFIEMEIEATNGAVPQGQRPPPRIKNFQINNQIVKLKYCYTCKIFRPPRASHCSICDNCVERFDHHCPWVGNCVGKRNYRYFYLFILSLSLLTIYVFAFNIVYVALKSLKIGFLETLKETPGTVLEVLICFFTLWSVVGLTGFHTFLVALNQTTNEDIKGSWTGKNRVQNPYSHGNIVKNCCEVLCGPLPPSVLDRRGILPLEESGSRPPSTQETSSSLLPQSPAPTEHLNSNEMPEDSSTPEEMPPPEPPEPPQEAAEAEK Predict reactive species |
| Full Name | zinc finger, DHHC-type containing 9 |
| Calculated Molecular Weight | 41 kDa |
| Observed Molecular Weight | 48 kDa |
| GenBank Accession Number | BC006200 |
| Gene Symbol | ZDHHC9 |
| Gene ID (NCBI) | 51114 |
| RRID | AB_3086522 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9Y397 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ZDHHC9, also named as CXorf11, ZDHHC10, ZNF379 or ZNF380, is a 364 amino acid protein, which contains one DHHC-type zinc finger and belongs to the DHHC palmitoyltransferase activity. ZDHHC9 localizes in the endoplasmic reticulum membrane and is highly expressed in kidney, skeletal muscle, brain, lung and liver. The ZDHHC9-GOLGA7 complex is a palmitoyltransferase specific for HRAS and NRAS.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ZDHHC9 antibody 82739-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ZDHHC9 Recombinant monoclonal antibody (9G5), catalog number 82739-1-RR, has 2 citations. These appear in Nature Communications and Scientific Reports. The cited research spans immunology/infectious disease (TBK1 palmitoylation in anti-malarial immunity) and cancer biology (ZDHHC9-driven osteosarcoma progression via RAS/MAPK signaling). Applications used include WB, IF, and CoIP across human and mouse samples.
| Species | Application | Title |
|---|---|---|
Nat Commun Palmitoylation of TBK1 enhances the type I interferon signaling and strengthens anti-malarial immunity in mice. | ||
Sci Rep ZDHHC9 promotes osteosarcoma progression via KRAS-associated enhancement of the RAS/MAPK signaling pathway.
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