Tested Applications
| Positive WB detected in | HEK-293T cells, Jurkat cells, HepG2 cells, HeLa cells, A431 cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84359-3-RR targets ZNF638 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag35296 Product name: Recombinant human ZNF638 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1777-1978 aa of NM_014497.4 Sequence: VDEVGEEEDGDNDLKVELAQSKNDHPTDKKGNRKKRAVDTKKTKLESLSQVGPVNENVMEEDLKTMIERHLTAKTPTKRVRIGKTLPSEKAVVTEPAKGEEAFQMSEVDEESGLKDSEPERKRKKTEDSSSGKSVASDVPEELDFLVPKAGFFCPICSLFYSGEKAMTNHCKSTRHKQNTEKFMAKQRKEKEQNEAEERSSR Predict reactive species |
| Full Name | zinc finger protein 638 |
| Calculated Molecular Weight | 221kDa |
| Observed Molecular Weight | 270-300 kDa |
| GenBank Accession Number | NM_014497.4 |
| Gene Symbol | ZNF638 |
| Gene ID (NCBI) | 27332 |
| RRID | AB_3671896 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | Q14966 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ZNF638 (Zinc finger protein 638) is also named as NP220 and ZFML. ZNF638 is a newly discovered member of zinc finger protein family for adipocyte function in vitro. And ZNF638regulates triglyceride metabolism via ANGPTL8 in an estrogen dependent manner (PMID: 38211696). ZNF638 plays a role as a transcriptional coregulator of adipocyte differentiation via induction of PPARγ in cooperation with CCAAT/enhancer binding proteins (C/EBPs) (PMID: 25024404). ZNF638 is localized in nuclear bodies enriched with splicing factors (PMID: 25024404).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ZNF638 antibody 84359-3-RR | Download protocol |
| WB protocol for ZNF638 antibody 84359-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
