Alpha Synuclein Monoclonal antibody
Alpha Synuclein Monoclonal Antibody for IHC, WB,ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, rat, mouse
Applications
WB, IHC,ELISA
Conjugate
Unconjugated
CloneNo.
1B10E9
Cat no : 66412-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse brain tissue, rat brain tissue |
Positive IHC detected in | mouse cerebellum tissue, mouse brain tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Sample-dependent, check data in validation data gallery |
Published Applications
IHC | See 1 publications below |
Product Information
66412-1-Ig targets Alpha Synuclein in WB, IHC,ELISA applications and shows reactivity with human, rat, mouse samples.
Tested Reactivity | human, rat, mouse |
Cited Reactivity | rat |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | Alpha Synuclein fusion protein Ag1285 |
Full Name | synuclein, alpha (non A4 component of amyloid precursor) |
Calculated molecular weight | 14 kDa |
Observed molecular weight | 18-19 kDa |
GenBank accession number | BC013293 |
Gene symbol | SNCA |
Gene ID (NCBI) | 6622 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Alpha Synuclein (α-syn) is a 14-19 kDa phosphoprotein that is primarily localize to the presynaptic terminals of mature neurons, where it is involved in synaptic function and plasticity. α-syn has drawn intense interest ever since the late 1990s, when the first α-synuclein missense mutation was identified as a cause of familial Parkinson's disease (PD). Aggregated and hyper-phosphorylated forms of α-syn protein are the pathological hallmark of Lewy body disease, which includes Parkinson's disease (PD), diffuse Lewy body disease (DLBD), and Lewy body variant of Alzheimer's disease (LBV). This antibody can recognize all the isoforms of α-syn.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for Alpha Synuclein antibody 66412-1-Ig | Download protocol |
IHC protocol for Alpha Synuclein antibody 66412-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Neuropathol Exp Neurol Regular Aerobic Exercise-Alleviated Dysregulation of CAMKIIα Carbonylation to Mitigate Parkinsonism via Homeostasis of Apoptosis With Autophagy. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Sheela (Verified Customer) (07-16-2019) | This product worked as expected
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