Alpha Synuclein Monoclonal antibody

Alpha Synuclein Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, rat, mouse

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

1B10E9

Cat no : 66412-1-Ig

Synonyms

Alpha synuclein, a-Synuclein, NACP, PARK1, PARK4, PD1, SNCA



Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inmouse cerebellum tissue, mouse brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Published Applications

IHCSee 1 publications below

Product Information

66412-1-Ig targets Alpha Synuclein in WB, IHC,ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Cited Reactivity rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Alpha Synuclein fusion protein Ag1285
Full Name synuclein, alpha (non A4 component of amyloid precursor)
Calculated molecular weight 14 kDa
Observed molecular weight 18-19 kDa
GenBank accession numberBC013293
Gene symbol SNCA
Gene ID (NCBI) 6622
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Alpha Synuclein (α-syn) is a 14-19 kDa phosphoprotein that is primarily localize to the presynaptic terminals of mature neurons, where it is involved in synaptic function and plasticity. α-syn has drawn intense interest ever since the late 1990s, when the first α-synuclein missense mutation was identified as a cause of familial Parkinson's disease (PD). Aggregated and hyper-phosphorylated forms of α-syn protein are the pathological hallmark of Lewy body disease, which includes Parkinson's disease (PD), diffuse Lewy body disease (DLBD), and Lewy body variant of Alzheimer's disease (LBV). This antibody can recognize all the isoforms of α-syn.

Protocols

Product Specific Protocols
WB protocol for Alpha Synuclein antibody 66412-1-IgDownload protocol
IHC protocol for Alpha Synuclein antibody 66412-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIHC

J Neuropathol Exp Neurol

Regular Aerobic Exercise-Alleviated Dysregulation of CAMKIIα Carbonylation to Mitigate Parkinsonism via Homeostasis of Apoptosis With Autophagy.

Authors - Wenfeng Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Sheela (Verified Customer) (07-16-2019)

This product worked as expected

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse brain lysate
Alpha Synuclein Antibody Western Blot, validation (1:1000 dilution) in Mouse brain lysate (Cat no:66412-1-Ig)