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c-SRC Antibody 6 Publications

Mouse Monoclonal| Catalog number: 60315-1-Ig |CloneNo.: 5E10C4

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Con: 150 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
Application not tested? Please see our Guarantee
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.


Images See all

c-SRC Antibody WB HeLa cells 60315-1-Ig

Western blot of c-SRC in various cell lines with 60315-1-Ig at dilution of 1:4000 incubated at room temperature for 1.5 hours

c-SRC Antibody IHC human breast cancer tissue 60315-1-Ig

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60315-1-Ig( c-SRC Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

c-SRC Antibody IHC human breast cancer tissue 60315-1-Ig

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60315-1-Ig( c-SRC Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

c-SRC Antibody IF MCF-7 cells 60315-1-Ig

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed MCF-7 cells using 60315-1-Ig(c-SRC antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L)

Species specificity:
human, hamster, mouse
Positive WB detected in:
HeLa cells, CHO cells, HEK-293 cells, RAW 264.7 cells
Positive IHC detected in:
human breast cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
MCF-7 cells
Recommended dilution:
WB : 1:1000-1:8000
IHC : 1:100-1:400
IF : 1:50-1:500
Sample-dependent, check data in Validation tab

Product Information

Source:
Mouse
Purification method:
Protein A purification
Isotype:
IgG2b
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
c-SRC fusion protein Ag22306
Full name:
v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)
Calculated molecular weight:
60 kDa
Observed molecular weight:
60 kDa
GenBank accession number:
BC011566
Gene ID (NCBI):
6714
Gene symbol
SRC
Synonyms
ASV, c SRC, c-SRC, p60 Src, pp60c src, Proto oncogene c Src, SRC, SRC1

Background

SRC, also named as SRC1 and p60-Src, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It is a non-receptor protein tyrosine kinase that plays pivotal roles in numerous cellular processes such as proliferation, migration, and transformation. In concert with PTK2B, SRC plays an important role in osteoclastic bone resorption. It promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase.


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