CoraLite®594-conjugated c-SRC Monoclonal antibody

c-SRC Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2b

Reactivity

human, hamster, mouse

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

5E10C4

Cat no : CL594-60315

Synonyms

ASV, c SRC, p60 Src, pp60c src, Proto oncogene c Src, SRC, SRC1



Tested Applications

Positive IF detected inMCF-7 cells, human breast cancer tissue
For other applications, we recommend the unconjugated version of this antibody, 60315-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-60315 targets c-SRC in IF applications and shows reactivity with human, hamster, mouse samples.

Tested Reactivity human, hamster, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen c-SRC fusion protein Ag22306
Full Name v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)
Calculated molecular weight 60 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC011566
Gene symbol SRC
Gene ID (NCBI) 6714
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths593 nm/614 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SRC, also named as SRC1 and p60-Src, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It is a non-receptor protein tyrosine kinase that plays pivotal roles in numerous cellular processes such as proliferation, migration, and transformation. In concert with PTK2B, SRC plays an important role in osteoclastic bone resorption. It promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase.

Protocols

Product Specific Protocols
IF protocol for CL594 c-SRC antibody CL594-60315Download protocol
Standard Protocols
Click here to view our Standard Protocols