"GST Tag Antibodies" Comparison
View side-by-side comparison of GST Tag antibodies from other vendors to find the one that best suits your research needs.
Tested Applications
| Positive WB detected in | Recombinant protein |
| Positive IF/ICC detected in | Transfected HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10000-0-AP targets GST Tag in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with recombinant protein samples.
| Tested Reactivity | recombinant protein |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Recombinant protein Predict reactive species |
| Full Name | GST Tag |
| Calculated Molecular Weight | 26 kDa |
| GenBank Accession Number | FN315687 |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_11042316 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GST(Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. Antibodies to GST are useful for checking protein expression both in plaques and on western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody can be used to detect the GST tagged protein. This antibody can be used as Rabbit IgG control in FC.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for GST Tag antibody 10000-0-AP | Download protocol |
| WB protocol for GST Tag antibody 10000-0-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther The noncanonical function of liver-type phosphofructokinase potentiates the efficacy of HDAC inhibitors in cancer. | ||
Signal Transduct Target Ther A dual-role of SARS-CoV-2 nucleocapsid protein in regulating innate immune response. | ||
Signal Transduct Target Ther SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis | ||
Signal Transduct Target Ther Visinin-like protein 1 disrupts calcium homeostasis and promotes atrial fibrillation in human and rodent models | ||
Signal Transduct Target Ther Seryl tRNA synthetase cooperates with POT1 to regulate telomere length and cellular senescence. | ||
Cancer Cell B cells disrupt tertiary lymphoid structure formation and suppress anti-tumor immunity. |
Reviews
What customers say
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Daniel (Verified Customer) (06-10-2022) | We use this anti-GST antibody for GST fusion protein-lipid interactions. We found this antibody gives much less background than other antibodies.
 |
Lana (Verified Customer) (04-06-2022) | SDS-PAGE: 4-12% Bis-Tris gradient gel. Transfer: Immobilon-FL transfer membranes (Millipore) O/N at 30V, 4C. Blocking: SEA Block Blocking Buffer 1h, room T. Primary Ab: O/N incubation at 4C, 1:1000. Secondary Ab: IRDye 680LT Goat anti-Rabbit, 1:15000. Lines of WB image: 1 – protein ladder, 2 – mitochondria fraction lysate, 3 – GST protein, 4 – GST fusion protein
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Shihab (Verified Customer) (10-28-2021) | Tried this antibody for a control experiment and worked nicely. Will be continuing to try this in immunofluorescence
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Amie (Verified Customer) (07-27-2021) | I compared this antibody directly to the proteintech mouse monoclonal (66001-2-Ig). In the provided image, the first 4 lanes of each gel are identical. The rabbit antibody seems to be slightly better (less background) than the mouse.
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Yan (Verified Customer) (02-20-2021) | It worked very well when I performed western blot after immunoprecipitation.
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