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NF-κB p65 Antibody 307 Publications

Rabbit Polyclonal| Catalog number: 10745-1-AP

Featured Product KD/KO validated

1 customer review

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Con: 78 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, chIP, ELISA
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Images See all

NF-κB p65 Antibody WB HEK-293 cells 10745-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 10745-1-AP (p65; RELA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

NF-κB p65 Antibody WB Hek-293 cells 10745-1-AP

WB result of RELA, p65antibody (10745-1-AP, 1:500) with si-Control and si-RELA,p65 transfected HeLa cells.

NF-κB p65 Antibody WB HEK-293 cells 10745-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 10745-1-AP (NF-κB p65 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

NF-κB p65 Antibody IP HeLa cells 10745-1-AP

IP Result of anti-p65 (IP:10745-1-AP, 3ug; Detection:10745-1-AP 1:1000) with HeLa cells lysate 5000ug.

NF-κB p65 Antibody IHC human breast cancer tissue 10745-1-AP

Immunohistochemical analysis of paraffin-embedded human breast cancer using 10745-1-AP(p65 antibody) at dilution of 1:100 (under 40x lens)

NF-κB p65 Antibody IF HepG2 cells 10745-1-AP

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HepG2 cells using 10745-1-AP(p65; RELA antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

NF-κB p65 Antibody FC HeLa cells 10745-1-AP

1X10^6 HeLa cells were stained with 0.2ug p65; RELA antibody (10745-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Species specificity:
human, mouse, rat, pig, A. sphaerocephala Krasch, CHICKEN, dog, hamster, hamsters, swine
Positive WB detected in:
HEK-293 cells, mouse liver tissue, MCF-7 cells, A431 cells, K-562 cells, NIH/3T3 cells, Jurkat cells, HeLa cells, mouse lung tissue, rat lung tissue
Positive IP detected in:
HeLa cells
Positive IHC detected in:
human breast cancer tissue, human liver cancer tissue, human stomach tissue, mouse heart tissue, mouse kidney tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells
Positive FC detected in:
HeLa cells
Recommended dilution:
WB : 1:1000-1:4000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:50-1:500
IF : 1:50-1:500
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
NF-κB p65 fusion protein Ag1199
Full name:
v-rel reticuloendotheliosis viral oncogene homolog A (avian)
Calculated molecular weight:
65 kDa
Observed molecular weight:
65 kDa
GenBank accession number:
BC011603
Gene ID (NCBI):
5970
Gene symbol
RELA
Synonyms
NFKB 3, NFKB p65, NFKB3, NF-κB3, p65, RELA, Transcription factor p65

Background

Nuclear factor k B (NF-kB) is a sequence-specific DNA-binding protein complex which regulates the expression of viral genomes, including the human immunodeficiency virus, and a variety of cellular genes, particularly those involved in immune and inflammatory responses. The members of the NF-kB family in mammalian cells include the proto-oncogene c-Rel,p50/p105 (NFkB1), p65 (RelA), p52/p100 (NFkB2), and RelB. All of these proteins share a conserved 300-amino acid region known as the Rel homology domain which is responsible for DNA binding, dimerization, and nuclear translocation of NF-kB. The p65 subunit is a major component of NF-kB complexes and is responsible for trans-activation. NF-kB heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kB p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of IkB upon NF-kB the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kB complex. It associates with chromatin at the NF-kB promoter region via association with DDX1. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human RELA.

Customer Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Share your scientific insight on this antibody

NF-κB p65 Rabbit Polyclonal antibody

Fraser (Verified Customer), 06-06-2018
Applications
Other (please state below),
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Lot Number
00017138

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