p65; RELA Antibody 132 Publications

Rabbit Polyclonal| Catalog number: 10745-1-AP

Featured Product KD/KO validated

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Freight/Packing

Con: 78 μg/150 μl

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Species specificity:
human, mouse, rat, pig, A. sphaerocephala Krasch, dog, hamsters

Positive WB detected in:
HeLa cells, A431 cells, HEK-293 cells, Jurkat cells, K-562 cells, MCF-7 cells, mouse liver tissue, mouse lung tissue, NIH/3T3 cells, pig liver tissue, rat kidney tissue, rat lung tissue

Positive IP detected in:
HeLa cells

Positive IHC detected in:
human breast cancer tissue, human liver cancer tissue, human stomach tissue

Positive IF detected in:
HepG2 cells,

Positive FC detected in:
HeLa cells

Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:50-1:500
IF : 1:50-1:500

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
v-rel reticuloendotheliosis viral oncogene homolog A (avian)

Calculated molecular weight:
65 kDa

Observed molecular weight:
65 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
RELA

Synonyms
NFKB 3, NFKB p65, NFKB3, p65, RELA, Transcription factor p65
Background

Nuclear factor k B (NF-kB) is a sequence-specific DNA-binding protein complex which regulates the expression of viral genomes, including the human immunodeficiency virus, and a variety of cellular genes, particularly those involved in immune and inflammatory responses. The members of the NF-Kb family in mammalian cells include the proto-oncogene c-Rel,p50/p105 (NFkB1), p65 (RelA), p52/p100 (NFkB2), and RelB. All of these proteins share a conserved 300-amino acid region known as the Rel homology domain which is responsible for DNA binding, dimerization, and nuclear translocation of NF-Kb. The p65 subunit is a major component of NF-Kb complexes and is responsible for trans-activation. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. It associates with chromatin at the NF-kappa-B promoter region via association with DDX1. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human RELA.


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