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  • KD/KO Validated

NF-κB p65 Antibody

NF-κB p65 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig and More (6)

Applications

FC, IF, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat no : 10745-1-AP

Synonyms

NFKB 3, NFKB p65, NFKB3, NF-κB3, p65, RELA, Transcription factor p65



Tested Applications

Positive WB detected inHEK-293 cells, mouse liver tissue, MCF-7 cells, A431 cells, K-562 cells, NIH/3T3 cells, Jurkat cells, HeLa cells, mouse lung tissue, rat lung tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue, human stomach tissue, mouse heart tissue, mouse kidney tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10745-1-AP targets NF-κB p65 in FC, IF, IHC, IP, WB, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Reactivity human, mouse, rat, pig
Cited Species A. sphaerocephala Krasch, CHICKEN, dog, hamster, hamsters, human, mouse, pig, rat, swine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NF-κB p65 fusion protein Ag1199
Full Name v-rel reticuloendotheliosis viral oncogene homolog A (avian)
Calculated molecular weight 65 kDa
Observed molecular weight 65 kDa
GenBank accession number BC011603
Gene symbol RELA
Gene ID (NCBI) 5970
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

Nuclear factor k B (NF-kB) is a sequence-specific DNA-binding protein complex which regulates the expression of viral genomes, including the human immunodeficiency virus, and a variety of cellular genes, particularly those involved in immune and inflammatory responses. The members of the NF-kB family in mammalian cells include the proto-oncogene c-Rel,p50/p105 (NFkB1), p65 (RelA), p52/p100 (NFkB2), and RelB. All of these proteins share a conserved 300-amino acid region known as the Rel homology domain which is responsible for DNA binding, dimerization, and nuclear translocation of NF-kB. The p65 subunit is a major component of NF-kB complexes and is responsible for trans-activation. NF-kB heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kB p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of IkB upon NF-kB the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kB complex. It associates with chromatin at the NF-kB promoter region via association with DDX1. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human RELA.

Protocols

Product Specific Protocols
WB protocol for NF-κB p65 antibody 10745-1-APDownload protocol
IHC protocol for NF-κB p65 antibody 10745-1-APDownload protocol
IF protocol for NF-κB p65 antibody 10745-1-APDownload protocol
IP protocol for NF-κB p65 antibody 10745-1-APDownload protocol
FC protocol for NF-κB p65 antibody 10745-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Food Nutr Res

Pretreatment with Lactobacillus reuteri F-9-35 attenuates ethanol-induced gastric injury in rats.

Authors - Mao-Cheng Sun
mouseWB

Pharmacol Res

Pharmacological preconditioning with the cellular stress inducer thapsigargin protects against experimental sepsis.

Authors - Yaping Wei
mouseWB

Front Pharmacol

Protective Effects of Costunolide Against D-Galactosamine and Lipopolysaccharide-Induced Acute Liver Injury in Mice.

Authors - Jingxin Mao
humanWB,chIP

J Exp Clin Cancer Res

Star-related lipid transfer protein 10 (STARD10): a novel key player in alcohol-induced breast cancer progression.

Authors - Andrea Floris
mouseWB

Front Cell Neurosci

IRAK-M Deficiency Exacerbates Ischemic Neurovascular Injuries in Experimental Stroke Mice.

Authors - Chenfei Lyu
humanWB

BMC Cancer

miR-196a-5p promotes metastasis of colorectal cancer via targeting IκBα.

Authors - He Xin

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Zehua Wang (12-17-2019)

After siRNA treatment for 48 hours, specific signal shown here. Works excellent!

  • Applications: Western Blot,Cell culture,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: K562
NF-κB p65 Antibody Western Blot,Cell culture, validation (1:1000 dilution) in K562 (Cat no:10745-1-AP)
FH

Feng-qian Li (12-07-2018)

  • Applications: Western Blot,Immunofluorescence,
  • Primary Antibody Dilution: 1/500
  • Cell Tissue Type: HaCaT
No image submitted with review
FH

Fraser Thomas (06-06-2018)

  • Applications: Other (please state below),
No image submitted with review