Name: NF-κB p65 Rabbit Polyclonal antibody
Brand: Proteintech
SKU: 10745-1-AP
Rating: 5.00 (1 reviews)

Tested Applications

Applications:

WB, IP, IHC, IF, FC, chIP, ELISA
Application not tested? Please see our Guarantee

NF-κB p65 Antibody WB HEK-293 cells 10745-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 10745-1-AP (p65; RELA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

WB result of RELA, p65antibody (10745-1-AP, 1:500) with si-Control and si-RELA,p65 transfected HeLa cells.

NF-κB p65 Antibody IP HeLa cells 10745-1-AP

IP Result of anti-p65 (IP:10745-1-AP, 3ug; Detection:10745-1-AP 1:1000) with HeLa cells lysate 5000ug.

NF-κB p65 Antibody IHC human breast cancer tissue 10745-1-AP

Immunohistochemical analysis of paraffin-embedded human breast cancer using 10745-1-AP(p65 antibody) at dilution of 1:100 (under 40x lens)

NF-κB p65 Antibody IF HepG2 cells 10745-1-AP

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HepG2 cells using 10745-1-AP(p65; RELA antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

NF-κB p65 Antibody FC HeLa cells 10745-1-AP

1X10^6 HeLa cells were stained with 0.2ug p65; RELA antibody (10745-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Species specificity:

human, mouse, rat, pig, A. sphaerocephala Krasch, CHICKEN, dog, hamster, hamsters, swine

Positive WB detected in:

HEK-293 cells, mouse lung tissue, MCF-7 cells, A431 cells, K-562 cells, NIH/3T3 cells, Jurkat cells, HeLa cells, mouse liver tissue

Positive IP detected in:

HeLa cells

Positive IHC detected in:

human breast cancer tissue, human liver cancer tissue, human stomach tissue, mouse heart tissue, mouse kidney tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Positive IF detected in:

HepG2 cells

Positive FC detected in:

HeLa cells

Recommended dilution:

WB : 1:1000-1:4000

IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB

IHC : 1:50-1:500

IF : 1:50-1:500

Sample-dependent, check data in Validation tab

Product Information

Source:

Rabbit

Purification method:

Antigen affinity purification

Isotype:

IgG

Storage:

PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20^oC. Aliquoting is unnecessary for -20^oC storage.

Immunogen Information

Immunogen:

NF-κB p65 fusion protein Ag1199

Full name:

v-rel reticuloendotheliosis viral oncogene homolog A (avian)

Calculated molecular weight:

65 kDa

Observed molecular weight:

65 kDa

GenBank accession number:

BC011603

Gene ID (NCBI):

5970

Gene symbol

RELA

Synonyms

NFKB 3, NFKB p65, NFKB3, NF-κB3, p65, RELA, Transcription factor p65

Background

Nuclear factor k B (NF-kB) is a sequence-specific DNA-binding protein complex which regulates the expression of viral genomes, including the human immunodeficiency virus, and a variety of cellular genes, particularly those involved in immune and inflammatory responses. The members of the NF-kB family in mammalian cells include the proto-oncogene c-Rel,p50/p105 (NFkB1), p65 (RelA), p52/p100 (NFkB2), and RelB. All of these proteins share a conserved 300-amino acid region known as the Rel homology domain which is responsible for DNA binding, dimerization, and nuclear translocation of NF-kB. The p65 subunit is a major component of NF-kB complexes and is responsible for trans-activation. NF-kB heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kB p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of IkB upon NF-kB the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kB complex. It associates with chromatin at the NF-kB promoter region via association with DDX1. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human RELA.

Customer Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Share your scientific insight on this antibody

NF-κB p65 Rabbit Polyclonal antibody

Fraser (Verified Customer), 06-06-2018

Applications

Other (please state below),

No image submitted with review

Lot Number

00017138

Showing 1- [6] of [284] Publications

Author:		Shuai Chen
Pubmed ID:		28358376
Journal:		Cell Death Dis
Title:		RelA/p65 inhibition prevents tendon adhesion by modulating inflammation, cell proliferation, and apoptosis.
Application:		WB
KD validated
Species:		human

Author:		Ding Li
Pubmed ID:		30559256
Journal:		Mol Cancer Res
Title:		NF-κB and poly (ADP-ribose) polymerase 1 form a positive-feedback loop that regulates DNA repair in acute myeloid leukemia cells.
Application:		WB
KD validated
Species:		human

Author:		Qin Wang
Pubmed ID:		28107661
Journal:		Biomaterials
Title:		Targeting NF-kB signaling with polymeric hybrid micelles that co-deliver siRNA and dexamethasone for arthritis therapy.
Application:		WB,IF
KD validated
Species:		mouse

Author:		Haiyan Xu
Pubmed ID:		28100608
Journal:		J Virol
Title:		Herpes Simplex Virus Type 1 UL24 Abrogates DNA Sensing Signal Pathway by inhibiting NF-κB Activation.
Application:		WB,IF

Species:		human

Author:		Daoyuan Ren
Pubmed ID:		28251195
Journal:		Food Funct
Title:		Chemical characterization of a novel polysaccharide ASKP-1 from Artemisia sphaerocephala Krasch seed and its macrophage activation via MAPK, PI3k/Akt and NF-κB signaling pathways in RAW264.7 cells.
Application:		WB

Species:		A. sphaerocephala Krasch

Author:		Xiaobao Gong
Pubmed ID:		28282576
Journal:		Int Immunopharmacol
Title:		Antioxidation, anti-inflammation and anti-apoptosis by paeonol in LPS/d-GalN-induced acute liver failure in mice.
Application:		WB

Species:		mouse

WB Figures

NF-κB p65 Antibody WB A431 cells 10745-1-AP

A431 cells were subjected to SDS PAGE followed by western blot with 10745-1-AP(p65 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

HEK-293 cells were subjected to SDS PAGE followed by western blot with 10745-1-AP(p65 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

WB result of RELA, p65antibody (10745-1-AP, 1:500) with si-Control and si-RELA,p65 transfected HeLa cells.

Various lysates were subjected to SDS PAGE followed by western blot with 10745-1-AP (p65; RELA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

NF-κB p65 Antibody WB HeLa cells 10745-1-AP

HeLa cells were subjected to SDS PAGE followed by western blot with 10745-1-AP(p65 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

NF-κB p65 Antibody WB Jurkat cells 10745-1-AP

Jurkat cells were subjected to SDS PAGE followed by western blot with 10745-1-AP(p65 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

NF-κB p65 Antibody WB K-562 cells 10745-1-AP

K-562 cells were subjected to SDS PAGE followed by western blot with 10745-1-AP(p65 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

NF-κB p65 Antibody WB MCF-7 cells 10745-1-AP

MCF7 cells were subjected to SDS PAGE followed by western blot with 10745-1-AP(p65 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

NF-κB p65 Antibody WB mouse liver tissue 10745-1-AP

mouse liver tissue were subjected to SDS PAGE followed by western blot with 10745-1-AP( p65; RELA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

NF-κB p65 Antibody WB mouse lung tissue 10745-1-AP

mouse lung tissue were subjected to SDS PAGE followed by western blot with 10745-1-AP(p65 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

NF-κB p65 Antibody WB NIH/3T3 cells 10745-1-AP

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10745-1-AP(p65 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

IHC Figures

Immunohistochemical analysis of paraffin-embedded human breast cancer using 10745-1-AP(p65 antibody) at dilution of 1:100 (under 10x lens)

Immunohistochemical analysis of paraffin-embedded human breast cancer using 10745-1-AP(p65 antibody) at dilution of 1:100 (under 40x lens)

NF-κB p65 Antibody IHC human liver cancer tissue 10745-1-AP

Immunohistochemical analysis of paraffin-embedded human liver cancer using 10745-1-AP(p65 antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemical analysis of paraffin-embedded human liver cancer using 10745-1-AP(p65 antibody) at dilution of 1:50 (under 40x lens)

NF-κB p65 Antibody IHC human stomach tissue 10745-1-AP

Immunohistochemical analysis of paraffin-embedded human stomach using 10745-1-AP(p65 antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemical analysis of paraffin-embedded human stomach using 10745-1-AP(p65 antibody) at dilution of 1:50 (under 40x lens)

IP Figures

IP Result of anti-p65 (IP:10745-1-AP, 3ug; Detection:10745-1-AP 1:1000) with HeLa cells lysate 5000ug.

IF Figures

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HepG2 cells using 10745-1-AP(p65; RELA antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

NF-κB p65 Antibody 284 Publications

Rabbit Polyclonal| Catalog number: 10745-1-AP

-_-

Tested Applications

Product Information

Immunogen Information

Background

Customer Reviews

NF-κB p65 Rabbit Polyclonal antibody

Showing 1- [6] of [284] Publications

WB Figures

IHC Figures

IP Figures

IF Figures

FC Figures

Product-specific protocols

Standard Protocols

NF-κB p65 Antibody 284 Publications

Rabbit Polyclonal| Catalog number: 10745-1-AP

-_-

Related Primary Antibodies

Special offer: Add control/tag antibody for $99

Tested Applications

Product Information

Immunogen Information

Background

Customer Reviews

NF-κB p65 Rabbit Polyclonal antibody