Adenosine A1 Receptor Polyklonaler Antikörper
Adenosine A1 Receptor Polyklonal Antikörper für IHC, WB, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus und mehr (1)
Anwendung
WB, IHC, IF, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 55026-1-AP
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | SH-SY5Y-Zellen |
| Erfolgreiche Detektion in IHC | Maus-Cerebellum-Gewebe, Maushirngewebe, Maushodengewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunhistochemie (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| WB | See 5 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Produktinformation
55026-1-AP bindet in WB, IHC, IF, ELISA Adenosine A1 Receptor und zeigt Reaktivität mit human, Maus
| Getestete Reaktivität | human, Maus |
| In Publikationen genannte Reaktivität | human, Maus, Ratte |
| Wirt / Isotyp | Kaninchen / IgG |
| Klonalität | Polyklonal |
| Typ | Antikörper |
| Immunogen | Peptid |
| Vollständiger Name | adenosine A1 receptor |
| Berechnetes Molekulargewicht | 37 kDa |
| Beobachtetes Molekulargewicht | 37 kDa |
| GenBank-Zugangsnummer | NM_000674 |
| Gene symbol | ADORA1 |
| Gene ID (NCBI) | 134 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Antigen-Affinitätsreinigung |
| Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
| Lagerungsbedingungen | Bei -20℃ lagern. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Adenosine A1 receptor (ADORA1, also known as A1AR) is one of four subtypes of adenosine receptors that belong to a superfamily of protein G-coupled receptors. Adenosine is produced in response to trauma, ischemia, and hypoxia and plays an important role in preventing excessive tissue damage.
What is the molecular weight of adenosine A1 receptor? Are there any isoforms of ADORA1?
The molecular weight of adenosine A1 receptor is 32-37 kDa. ADORA1 forms homodimers that can be stable in denaturing condition running as a ~64 kDa species in western blotting (PMID: 19558453). Alternative splicing resulting in two isoforms has been reported (PMID: 8300646).
What is the tissue expression of adenosine A1 receptor? What is the subcellular localization of ADORA1?
Adenosine A1 receptor is expressed in various organs, with very high expression in the central nervous system (mainly in the cortex, cerebellum, and hippocampus but also by microglia, astrocytes, and oligodendrocytes), adrenal gland, and atrium (PMID: 11734617). Adenosine receptors, including A1 receptor, are integral membrane proteins that reside at the plasma membrane.
What is the role of adenosine A1 receptor in health and disease?
Adenosine is an important neuromodulator influencing synaptic plasticity and the release of neurotransmitters, in addition to working as a neuroprotective agent against oxidative stress - all of these functions are mediated by adenosine A1 receptor, whose expression levels are increased upon stress. Adenosine A1 receptor has been shown to control sleep; ADORA1 levels increase during sleep deprivation and promote sleep (PMID: 9157887, 17329439). Additionally, adenosine A1 receptor regulates vascular tension by contraction, antagonizing the activity of adenosine A2 receptor (PMID: 15539423). A number of selective agonists and antagonists of adenosine receptors have been developed to study their biology and are now drug candidates in the clinic (PMID: 19639277).
Protokolle
| Produktspezifische Protokolle | |
|---|---|
| WB protocol for Adenosine A1 Receptor antibody 55026-1-AP | Protokoll herunterladen |
| IHC protocol for Adenosine A1 Receptor antibody 55026-1-AP | Protokoll herunterladen |
| Standard-Protokolle | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Antioxidants (Basel) Activation of Adenosine A1 Receptor in Ischemic Stroke: Neuroprotection by Tetrahydroxy Stilbene Glycoside as an Agonist. | ||
J Nutr Biochem Disruption of hepatic one-carbon metabolism impairs mitochondrial function and enhances macrophage activity in methionine-choline-deficient mice. | ||
Bioorg Med Chem Unraveling the mechanism of alkaloids from Sophora alopecuroides Linn combined with immune checkpoint blockade in the treatment of non-small cell lung cancer based on systems pharmacology. | ||
Anat Rec (Hoboken) Adenosine A1 Receptor mRNA Expression by Neurons and Glia in the Auditory Forebrain. | ||
J Ophthalmic Vis Res Adenosine Receptors Expression in Human Retina and Choroid with Age-related Macular Degeneration |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Brandon (Verified Customer) (10-31-2019) | 40 ug protein was transferred to a PVDF membrane and blocked in 5% milk in TBST for 1 hour before overnight incubation with 1' antibody diluted in 1% milk in TBST. Anti-rabbit HRP secondary antibody diluted 1:3000 in 5% milk was used for detection by chemiluminescence.The band did not show up when transferred to a nitrocellulose membrane and I noticed several non-specific bands
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