ALCAM Monoklonaler Antikörper

ALCAM Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

human

Anwendung

FC

Konjugation

CoraLite® Plus 405 Fluorescent Dye

CloneNo.

3A6

Kat-Nr. : CL405-65223

Synonyme

ALCAM, CD166, CD166 antigen, FLJ38514, MEMD

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  FC experiment of human PBMCs using CL405-65223

  1X10^6 PHA-treated (day 3) human PBMCs were surface stained with 5 ul CoraLite® Plus 405 Anti-Human ALCAM (CL405-65223, Clone:3A6) (red), or unstained (blue). The black dashed line indicates unactivated PBMCs surface stained with 5ul CoraLite® Plus 405 Anti-Human ALCAM. Cells were not fixed. Monocytes were gated.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: PHA-treated human PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

CL405-65223 bindet in FC ALCAM und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Cultured human thymic epithelial cells
• Vollständiger Name: activated leukocyte cell adhesion molecule
• Berechnetes Molekulargewicht: 105 kDa
• GenBank-Zugangsnummer: BC057809
• Gene symbol: ALCAM
• Gene ID (NCBI): 214
• Konjugation: CoraLite® Plus 405 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 399 nm / 422 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Activated leukocyte cell adhesion molecule (ALCAM, also known as CD166) is a cell adhesion molecule that belongs to the immunoglobulin superfamily. It is involved in cell-cell adhesion through homophilic and heterophilic (to CD6) interactions. ALCAM is widely expressed in a variety of normal tissues and cell types, including activated T cells and monocytes, epithelial cells, fibroblasts, neuronal cells, hepatocytes, and bone marrow mesenchymal stem cells (PMID: 7760007; 25221999). Altered ALCAM expression has been associated with the differentiation state and progression in some neoplasms including melanoma, prostate, colorectal, and breast cancers (PMID: 20461761; 18172759).