ASAH1 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

ASAH1 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

1B7E4

Rezensionen (1)

Kat-Nr. : 67092-1-Ig

Synonyme

AC, Acid ceramidase, Acylsphingosine deacylase, ASAH, ASAH1, FLJ21558, FLJ22079, HSD 33, PHP, PHP32, Putative 32 kDa heart protein

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Galerie der Validierungsdaten

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  WB analysis of rat cerebellum using 67092-1-Ig

  rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 67092-1-Ig (ASAH1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 67092-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67092-1-Ig (ASAH1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 67092-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67092-1-Ig (ASAH1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig cerebellum using 67092-1-Ig

  pig cerebellum tissue were subjected to SDS PAGE followed by western blot with 67092-1-Ig (ASAH1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 67092-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67092-1-Ig (ASAH1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67092-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67092-1-Ig (ASAH1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of SH-SY5Y using 67092-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using 67092-1-Ig (ASAH1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Ratten-Cerebellum-Gewebe, Hausschwein-Cerebellum-Gewebe, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

67092-1-Ig bindet in WB, IP, IHC, IF, ELISA ASAH1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ASAH1 fusion protein Ag28645
• Vollständiger Name: N-acylsphingosine amidohydrolase (acid ceramidase) 1
• Berechnetes Molekulargewicht: 44 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC016828
• Gene symbol: ASAH1
• Gene ID (NCBI): 427
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ASAH1, also named as AC, ACDase, Acid CDase, PHP32 and ASAH, belongs to the acid ceramidase family. ASAH1 is a lipid hydrolase that catalyzes the conversion of ceramide (cer) into sphingosine (SPH) and a free fatty acid. Mutation of ASAH1 will cause the Farber lipogranulomatosis (FL) which also known as Farber disease (FD). ASAH1 is highly expressed in heart. And it is first synthesized as an 55-60 kDa precursor and subsequently processed to the mature, heterodimeric enzyme (40 + 13 kDa) in the endosomes/lysosomes.

Publikationen

Species	Application	Title
mouse	WB,IF,IP	Mol Med; Acid ceramidase targeting pyruvate kinase affected trypsinogen activation in acute pancreatitis; Authors - Juan Xiao ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hua (Verified Customer) (01-20-2023)

Good antibody. May not be able to use on certain mouse tissues.

• Applications: Western Blot
• Primary Antibody Dilution: 1:4000
• Cell Tissue Type: Mouse brain

ASAH1 Antibody Western Blot validation (1:4000 dilution) in Mouse brain (Cat no:67092-1-Ig)