Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, MCF-7 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
29450-1-AP targets BRCA2 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag31064 Product name: Recombinant human BRCA2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 210-388 aa of NM_000059 Sequence: IVRNEEASETVFPHDTTANVKSYFSNHDESLKKNDRFIASVTDSENTNQREAASHGFGKTSGNSFKVNSCKDHIGKSMPNVLEDEVYETVVDTSEEDSFSLCFSKCRTKNLQKVRTSKTRKKIFHEANADECEKSKNQVKEKYSFVSEVEPNDTDPLDSNVANQKPFESGSDKISKEVV Predict reactive species |
| Full Name | breast cancer 2, early onset |
| Calculated Molecular Weight | 384 kDa |
| Observed Molecular Weight | 384 kDa |
| GenBank Accession Number | NM_000059 |
| Gene Symbol | BRCA2 |
| Gene ID (NCBI) | 675 |
| RRID | AB_2918309 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P51587 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
BRCA2, also named as FACD and FANCD1, is involved in double-strand break repair and/or homologous recombination. BRCA2 may participate in S phase checkpoint activation. Defects in BRCA2 are a cause of genetic susceptibility to breast cancer (BC) which also called susceptibility to familial breast-ovarian cancer type 2 (BROVCA2).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for BRCA2 antibody 29450-1-AP | Download protocol |
| WB protocol for BRCA2 antibody 29450-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The BRCA2 Polyclonal antibody (Cat# 29450-1-AP) has 17 citations. These appear in journals including Molecular Cell, Nucleic Acids Research, Oncogene, Cell Reports, Cancer Gene Therapy, International Journal of Molecular Sciences, American Journal of Physiology, Scientific Reports, PLoS ONE, Prostate, Oncology Letters, Aging, and International Journal of Surgery. The research fields span DNA replication stress, homologous recombination, DNA damage repair, and cancer biology (breast, ovarian, pancreatic, prostate, lung, bladder, and renal cancers), with emphasis on PARP inhibitor resistance and replication fork stalling.
| Species | Application | Title |
|---|---|---|
Mol Cell RAD51 succinylation regulates homologous recombination and contributes to the chemosensitivity in cancer. | ||
Nucleic Acids Res CSB and SMARCAL1 compete for RPA32 at stalled forks and differentially control the fate of stalled forks in BRCA2-deficient cells | ||
Oncogene KPNA2 silencing sensitizes triple-negative breast cancer to chemotherapy by promoting multipolar division and suppressing DNA damage repair. | ||
Oncogene The splicing factor SNRPB promotes ovarian cancer progression through regulating aberrant exon skipping of POLA1 and BRCA2 | ||
Cell Rep Ski2-like helicase ASCC3 unwinds DNA upon fork stalling to control replication stress responses. |
