Caspase 3(human specific) Monoklonaler Antikörper

Caspase 3(human specific) Monoklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human und mehr (4)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

66

CloneNo.

1E7F9

Kat-Nr. : 66470-1-Ig

Synonyme

Apopain, CASP 3, CASP3, Caspase 3, Caspase3, CPP 32, CPP32, CPP32B, Cysteine protease CPP32, Protein Yama, SCA 1, SREBP cleavage activity 1

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 66470-1-Ig

  Untreated and Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 66470-1-Ig (CASP3(human specific) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66470-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66470-1-Ig (CASP3(human specific) antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66470-1-Ig

  Untreated and staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 66470-1-Ig (CASP3(human specific) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 66470-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66470-1-Ig (CASP3(human specific) antibody at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66470-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66470-1-Ig (CASP3(human specific) antibody at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 66470-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66470-1-Ig (Caspase 3(human specific) antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 62 publications below
• IHC: See 2 publications below
• IF: See 4 publications below

Produktinformation

66470-1-Ig bindet in WB, IHC, IF, ELISA Caspase 3(human specific) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Zebrafisch, Kuh
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Caspase 3(human specific) fusion protein Ag25029
• Vollständiger Name: caspase 3, apoptosis-related cysteine peptidase
• Berechnetes Molekulargewicht: 277 aa, 32 kDa
• Beobachtetes Molekulargewicht: 32-35 kDa, 19 kDa, 17 kDa
• GenBank-Zugangsnummer: BC016926
• Gene symbol: CASP3
• Gene ID (NCBI): 836
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Casp3 (caspase 3), also named as CPP32, SCA-1 and Apopain, belongs to the peptidase C14A family. Caspase 3 is involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Caspase 3 cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. CASP3 is involved in the cleavage of huntingtin. This antibody is specific for human caspase 3, it can recognize the 32 kDa pro-caspase 3 as well as 17 and 19 kDa cleaved-caspase 3.

Publikationen

Species	Application	Title
human	WB	Sci Adv; The deacetylation-phosphorylation regulation of SIRT2-SMC1A axis as a mechanism of antimitotic catastrophe in early tumorigenesis.; Authors - Fei Yi
rat	WB	Redox Biol; Restoration of L-OPA1 alleviates acute ischemic stroke injury in rats via inhibiting neuronal apoptosis and preserving mitochondrial function.; Authors - Yongxing Lai
human	WB	Environ Int; Chemical conjugation of FITC to track silica nanoparticles in vivo and in vitro: An emerging method to assess the reproductive toxicity of industrial nanomaterials.; Authors - Junxia Li
human	WB	Cancer Lett; UM-6 induces autophagy and apoptosis via the Hippo-YAP signaling pathway in cervical cancer.; Authors - Dongying Wang
rat	WB,IHC	Oxid Med Cell Longev; Gastrodin Promotes the Survival of Random-Pattern Skin Flaps via Autophagy Flux Stimulation.; Authors - Hongyu Chen
human	WB	Oxid Med Cell Longev; Farrerol Enhances Nrf2-Mediated Defense Mechanisms against Hydrogen Peroxide-Induced Oxidative Damage in Human Retinal Pigment Epithelial Cells by Activating Akt and MAPK.; Authors - Ning Ma