CD107a / LAMP1 Monoklonaler Antikörper

CD107a / LAMP1 Monoklonal Antikörper für IF/ICC, FC, FC (Intra)

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

human

Anwendung

IF/ICC, FC, FC (Intra)

Konjugation

FITC Plus Fluorescent Dye

CloneNo.

H4A3

Kat-Nr. : FITC-65051

Synonyme

CD107a, LAMP1, LAMP-1, LAMP 1, H4A3



Geprüfte Anwendungen

Erfolgreiche Detektion in IF/ICCHeLa-Zellen
Erfolgreiche Detektion in FC (Intra)HeLa-Zellen
Erfolgreiche Detektion in FChumane PBMCs

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:500
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

FITC-65051 bindet in IF/ICC, FC, FC (Intra) CD107a / LAMP1 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgG1, kappa
Klonalität Monoklonal
Typ Antikörper
Immunogen Humane adhärente periphere Blutzellen
Vollständiger Name lysosomal-associated membrane protein 1
Berechnetes Molekulargewicht45 kDa
GenBank-ZugangsnummerBC006345
Gene symbol LAMP1
Gene ID (NCBI) 3916
Konjugation FITC Plus Fluorescent Dye
Excitation/Emission maxima wavelengths495 nm / 524 nm
Form Liquid
Reinigungsmethode Affinitätsreinigung
Lagerungspuffer PBS with 0.09% sodium azide and 0.5% BSA
LagerungsbedingungenStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

LAMP1 (CD107a) is a heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP1 is expressed largely in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane (PMID: 16168398). Elevated LAMP1 expression at the cell surface has also been detected during platelet and granulocytic cell activation, as well as in some tumor cells (PMID: 29085473). LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis (PMID: 18835598; 29085473; 9426697).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
IF protocol for FITC Plus CD107a / LAMP1 antibody FITC-65051Protokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen