CD107a / LAMP1 Monoklonaler Antikörper
CD107a / LAMP1 Monoklonal Antikörper für IF/ICC, FC (Intra)
Wirt / Isotyp
Maus / IgG1, kappa
Getestete Reaktivität
human
Anwendung
IF/ICC, FC (Intra)
Konjugation
CoraLite® Plus 647 Fluorescent Dye
CloneNo.
H4A3
Kat-Nr. : CL647-65051
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in IF/ICC | HeLa-Zellen |
| Erfolgreiche Detektion in FC (Intra) | HeLa-Zellen |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Immunfluoreszenz (IF)/ICC | IF/ICC : 1:200-1:800 |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| IF | See 2 publications below |
Produktinformation
CL647-65051 bindet in IF/ICC, FC (Intra) CD107a / LAMP1 und zeigt Reaktivität mit human
| Getestete Reaktivität | human |
| In Publikationen genannte Reaktivität | human |
| Wirt / Isotyp | Maus / IgG1, kappa |
| Klonalität | Monoklonal |
| Typ | Antikörper |
| Immunogen | Humane adhärente periphere Blutzellen |
| Vollständiger Name | lysosomal-associated membrane protein 1 |
| Berechnetes Molekulargewicht | 45 kDa |
| GenBank-Zugangsnummer | BC006345 |
| Gene symbol | LAMP1 |
| Gene ID (NCBI) | 3916 |
| Konjugation | CoraLite® Plus 647 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 654 nm / 674 nm |
| Form | Liquid |
| Reinigungsmethode | Protein-G-Reinigung |
| Lagerungspuffer | PBS with 0.09% sodium azide and 0.5% BSA |
| Lagerungsbedingungen | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Hintergrundinformationen
LAMP1 (CD107a) is a heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP1 is expressed largely in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane (PMID: 16168398). Elevated LAMP1 expression at the cell surface has also been detected during platelet and granulocytic cell activation, as well as in some tumor cells (PMID: 29085473). LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis (PMID: 18835598; 29085473; 9426697).
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| IF protocol for CL Plus 647 CD107a / LAMP1 antibody CL647-65051 | Protokoll herunterladen |
| FC protocol for CL Plus 647 CD107a / LAMP1 antibody CL647-65051 | Download protocol |
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Nat Commun EGFR core fucosylation, induced by hepatitis C virus, promotes TRIM40-mediated-RIG-I ubiquitination and suppresses interferon-I antiviral defenses | ||
J Zhejiang Univ Sci B Fibroblast growth factor 21 (FGF21) attenuates tacrolimus-induced hepatic lipid accumulation through transcription factor EB (TFEB)-regulated lipophagy |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Patrick (Verified Customer) (03-26-2025) | Nice bright signal worked really well for imaging flow cytometry
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