CD117 Monoklonaler Antikörper

CD117 Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

human, Rind

Anwendung

FC

Konjugation

APC Fluorescent Dye

CloneNo.

104D2

Kat-Nr. : APC-65154

Synonyme

C Kit, CD117, KIT, PBT, Proto oncogene c Kit, SCFR, Tyrosine protein kinase Kit



Geprüfte Anwendungen

Erfolgreiche Detektion in FCTF-1-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 10 µl per 10^6 cells in a 100 µl suspension or 10 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

APC-65154 bindet in FC CD117 und zeigt Reaktivität mit human, Rind

Getestete Reaktivität human, Rind
Wirt / Isotyp Maus / IgG1, kappa
Klonalität Monoklonal
Typ Antikörper
Immunogen MOLM-1 megakaryozytische Zellen
Vollständiger Name v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
Berechnetes Molekulargewicht 976 aa, 110 kDa
GenBank-ZugangsnummerBC071593
Gene symbol KIT
Gene ID (NCBI) 3815
Konjugation APC Fluorescent Dye
Excitation/Emission maxima wavelengths650 nm / 660 nm
Form Liquid
Reinigungsmethode Der gereinigte Antikörper ist mit Allophycocyanin (APC) unter optimalen Bedingungen konjugiert. Das Konjugat wird mit Größenausschluss-Chromatographie gereinigt.
Lagerungspuffer PBS mit 0,1% Natriumazid.
LagerungsbedingungenStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD117, also known as c-Kit and SCFR, is a transmembrane protein with tyrosine kinase activity encoded by the oncogene c-kit (PMID: 2448137). It is a member of the type III receptor tyrosine kinase family, which also includes CSF-1R, PDGFRβ, PDGFRα, and FLT3 (PMID: 29518044). CD117 is expressed on hematopoietic stem cells and progenitor cells, mast cells, and is also found in a wide range of non-haemopoietic cell types (including melanocytes, germ cells, astrocytes, renal tubules, breast glandular epithelial cells, sweat glands, and interstitial cells of Cajal) (PMID: 10582338; 23073628). CD117 plays an important role in early haemopoiesis. It is also involved in pigmentation, fertility, gut movement, and some aspects of the nervous system (PMID: 23073628).

Protokolle

Produktspezifische Protokolle
FC protocol for APC CD117 antibody APC-65154Protokoll herunterladen
Standard-Protokolle
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