CoraLite®594 Anti-Human CD117/c-Kit Rabbit Recombinant Antibody

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  FC experiment of human blood cells using CL594-98377

  1x10^6 human blood cells were surface stained with FITC Plus Anti-Human CD34, and 5 ul CoraLite®594 Anti-Human C-Kit Rabbit RecAb (CL594-98377, Clone: 241313C5) or CoraLite®594 Rabbit IgG Isotype Control RecAb (CL594-98136, Clone: 240953C9). Cells were not fixed. Lymphocytes were gated.

CD117/c-Kit Uni-rAb^® Recombinant Antibody for FC

Cat No. CL594-98377

Clone No.241313C5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CD117, KIT, C Kit, c-Kit, EC:2.7.10.1

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Tested Applications

• Positive FC detected in: human blood cells

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in a 100 µl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-98377 targets CD117/c-Kit in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
• Calculated Molecular Weight: 976 aa, 110 kDa
• GenBank Accession Number: BC071593
• Gene Symbol: CD117
• Gene ID (NCBI): 3815
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P10721
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD117, also known as c-Kit and SCFR, is a transmembrane protein with tyrosine kinase activity encoded by the oncogene c-kit (PMID: 2448137). It is a member of the type III receptor tyrosine kinase family, which also includes CSF-1R, PDGFRβ, PDGFRα, and FLT3 (PMID: 29518044). CD117 is expressed on hematopoietic stem cells and progenitor cells, mast cells, and is also found in a wide range of non-haemopoietic cell types (including melanocytes, germ cells, astrocytes, renal tubules, breast glandular epithelial cells, sweat glands, and interstitial cells of Cajal) (PMID: 10582338; 23073628). CD117 plays an important role in early haemopoiesis. It is also involved in pigmentation, fertility, gut movement, and some aspects of the nervous system (PMID: 23073628).