CD11b Monoklonaler Antikörper

CD11b Monoklonal Antikörper für FC

Wirt / Isotyp

Ratte / IgG2b, kappa

Getestete Reaktivität

human, Maus

Anwendung

IF, FC

Konjugation

FITC Plus Fluorescent Dye

Publikationen(14)

CloneNo.

M1/70

Kat-Nr. : FITC-65055

Synonyme

CR3, CR3A, integrin alpha M, Itgam, Ly 40

---

• 

  FC experiment of mouse bone marrow cells using FITC-65055

  1x10^6 mouse bone marrow cells were surface stained with CoraLite® Plus 647 Anti-Mouse Ly-6G/Ly-6C (Gr-1), and 0.25 ug FITC Plus Anti-Mouse CD11b (M1/70) (FITC-65055, Clone: M1/70) or FITC Plus Rat IgG2b Isotype Control (LTF-2) (FITC-65211, Clone: LTF-2). Cells were not fixed.

• 

  FC experiment of mouse bone marrow cells using FITC-65055

  1x10^6 mouse bone marrow cells were surface stained with 0.25 ug FITC Plus Anti-Mouse CD11b (M1/70) (FITC-65055, Clone: M1/70) (red) or FITC Plus Rat IgG2b Isotype Control (LTF-2) (FITC-65211, Clone: LTF-2) (blue). Cells were not fixed.

• 

  FC experiment of human PBMCs using FITC-65055

  1X10^6 human PBMCs were surface stained with 0.25 ug FITC Plus Anti-Mouse CD11b (FITC-65055, Clone: M1/70). Cells were not fixed. Monocytes were gated.

• 

  FC experiment of human PBMCs using FITC-65055

  1X10^6 human PBMCs were surface stained with 0.25 ug FITC Plus Anti-Mouse CD11b (FITC-65055, Clone:M1/70). Cells were not fixed. Monocytes were gated.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Knochenmarkzellen, humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 3 publications below
• FC: See 10 publications below

Produktinformation

FITC-65055 bindet in IF, FC CD11b und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Ratte / IgG2b, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: C57BL/10 Mausmilz-T-Zellen und Concanavalin A-aktivierte C57BL/10-Splenozyten
• Vollständiger Name: integrin alpha M
• Berechnetes Molekulargewicht: 127 kDa
• GenBank-Zugangsnummer: BC156991
• Gene symbol: CD11b
• Gene ID (NCBI): 16409
• Konjugation: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).

Publikationen

Species	Application	Title
		Cell Chem Biol; Small-molecule MHC-II inducers promote immune detection and anti-cancer immunity via editing cancer metabolism; Authors - Ling Huang
mouse	FC	Cell Commun Signal; Transfer of cGAMP from neuron to microglia activates microglial type I interferon responses after subarachnoid hemorrhage; Authors - Hanxiao Chang
mouse	FC	Cell Death Discov; TRAF6 triggers Mycobacterium-infected host autophagy through Rab7 ubiquitination; Authors - Qinmei Ma
		Neoplasia; Tumor microenvironment(TME) and single-source dual-energy CT(ssDECT) on assessment of inconformity between RECIST1.1 and pathological remission in neoadjuvant immunotherapy of NSCLC; Authors - Chao Sun
mouse	IF,FC	Int Immunopharmacol; Methionine enkephalin inhibited cervical carcinoma via apoptosis promotion and reduction of myeloid derived suppressor cell infiltrated in tumor.; Authors - Na Qu
mouse	FC	Cell Death Dis; Lactate secreted by PKM2 upregulation promotes Galectin-9-mediated immunosuppression via inhibiting NF-κB pathway in HNSCC.; Authors - Hanyue Chang