CD11b Monoklonaler Antikörper

CD11b Monoklonal Antikörper für FC

Wirt / Isotyp

Ratte / IgG2b, kappa

Getestete Reaktivität

human, Maus

Anwendung

IF, FC

Konjugation

FITC Plus Fluorescent Dye

Publikationen

5

CloneNo.

M1/70

Kat-Nr. : FITC-65055

Synonyme

CD11b, CD11b/CD18, CR3, CR3A, F730045J24Rik, integrin alpha M, Itgam, Ly 40, Mac 1, Mac 1a, MAC1

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Galerie der Validierungsdaten

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  FC experiment of mouse bone marrow cells using FITC-65055

  1X10^6 mouse bone marrow cells were surface stained with 0.25 ug FITC Plus Anti-Mouse CD11b (FITC-65055, Clone:M1/70) or unstained. Cells were not fixed.

• 

  FC experiment of human PBMCs using FITC-65055

  1X10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Mouse CD11b (FITC-65055, Clone: M1/70). Cells were not fixed. Monocytes were gated.

• 

  FC experiment of human PBMCs using FITC-65055

  1X10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Mouse CD11b (FITC-65055, Clone:M1/70). Cells were not fixed. Monocytes were gated.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Knochenmarkzellen, humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 2 publications below
• FC: See 4 publications below

Produktinformation

FITC-65055 bindet in IF, FC CD11b und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2b, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: C57BL/10 Mausmilz-T-Zellen und Concanavalin A-aktivierte C57BL/10-Splenozyten
• Vollständiger Name: integrin alpha M
• GenBank-Zugangsnummer: BC156991
• Gene symbol: Itgam
• Gene ID (NCBI): 16409
• Konjugation: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).

Publikationen

Species	Application	Title
mouse	IF,FC	Int Immunopharmacol; Methionine enkephalin inhibited cervical carcinoma via apoptosis promotion and reduction of myeloid derived suppressor cell infiltrated in tumor.; Authors - Na Qu
mouse	FC	Cell Death Dis; Lactate secreted by PKM2 upregulation promotes Galectin-9-mediated immunosuppression via inhibiting NF-κB pathway in HNSCC.; Authors - Hanyue Chang
mouse	IF	Molecules; Tabersonine Inhibits the Lipopolysaccharide-Induced Neuroinflammatory Response in BV2 Microglia Cells via the NF-κB Signaling Pathway; Authors - Jiaotai Shi
mouse	FC	Vet Res; Vimentin affects inflammation and neutrophil recruitment in airway epithelium during Streptococcus suis serotype 2 infection; Authors - Yu Meng
mouse	FC	ACS Nano; Bioengineered Nanospores Selectively Blocking LC3-Associated Phagocytosis in Tumor-Associated Macrophages Potentiate Antitumor Immunity; Authors - Yunkai Shang