Anti-Mouse CD11b (M1/70) Rat Recombinant Antibody

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  IF Staining of RAW 264.7 using 65672-1-TR

  Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using CD11b antibody (65672-1-TR, Clone: M1/70 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of mouse bone marrow cells using 65672-1-TR

  1x10^6 mouse bone marrow cells were surface stained with 0.5 ug Anti-Mouse CD11b (M1/70) Rat IgG2a RecAb (65672-1-TR, Clone: M1/70) or Rat IgG2a Isotype Control Recombinant Antibody (98427-1-TR, Clone: 250862A1), and FITC anti-Rat IgG2a antibody. Cells were co-stained with CoraLite® Plus 647 Anti-Mouse Ly-6G (CL647-98284: 242141B11). Cells were not fixed.

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  FC experiment of mouse bone marrow cells using 65672-1-TR

  1x10^6 mouse bone marrow cells were surface stained with 0.5 ug Anti-Mouse CD11b (M1/70) Rat IgG2a RecAb (65672-1-TR, Clone: M1/70) (red) or Rat IgG2a Isotype Control Recombinant Antibody (98427-1-TR, Clone: 250862A1) (blue), and FITC anti-Rat IgG2a antibody. Cells were not fixed.

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  FC experiment of rabbit splenocytes using 65672-1-TR

  1x10^6 rabbit splenocytes were surface stained with 0.25 ug Anti-Mouse CD11b (M1/70) Rat IgG2a RecAb (65672-1-TR, Clone:M1/70) or Rat IgG2a Isotype Control (2A3) (65209-1-Ig, Clone: 2A3), and CoraLite®750 anti-Rat IgG2a. Cells were co-stained with CoraLite® Plus 488 Anti-Rabbit CD3. Cells were not fixed.

CD11b Uni-rAb^® Recombinant Antibody for IF/ICC, FC

Cat No. 65672-1-TR

Clone No.M1/70

Host / Isotype

Rat / IgG2a

Reactivity

human, mouse, rabbit, monkey, guinea pig, non-human primates

Applications

IF/ICC, FC

Synonyms

CR3, CR3A, integrin alpha M, Itgam, Ly 40

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Tested Applications

• Positive IF/ICC detected in: RAW 264.7 cells
• Positive FC detected in: mouse bone marrow cells, rabbit splenocytes

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600
• Flow Cytometry (FC): FC : 0.5 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

65672-1-TR targets CD11b in IF/ICC, FC applications and shows reactivity with human, mouse, rabbit, monkey, guinea pig, non-human primates samples.

• Tested Reactivity: human, mouse, rabbit, monkey, guinea pig, non-human primates
• Host / Isotype: Rat / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen:

  N/A

• Full Name: integrin alpha M
• Calculated Molecular Weight: 127 kDa
• GenBank Accession Number: BC156991
• Gene Symbol: CD11b
• Gene ID (NCBI): 16409
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: E9Q604
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).