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CD151 Polyklonaler Antikörper

CD151 Polyklonal Antikörper für FC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, FC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10418-1-AP

Synonyme

CD151, CD151 antigen, GP27, Membrane glycoprotein SFA 1, MER2, PETA 3, RAPH, SFA1, Tetraspanin 24, Tspan 24, TSPAN24

Galerie der Validierungsdaten

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  • Western Blot (WB) analysis of various lysates using CD151 Polyclonal antibody (10418-1-AP)

    WB analysis using 10418-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10418-1-AP (CD151 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of A549 cells using CD151 Polyclonal antibody (10418-1-AP)

    FC experiment of A549 using 10418-1-AP

    1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human CD151 (10418-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Geprüfte Anwendungen

Erfolgreiche Detektion in WBA431-Zellen, A549-Zellen, Mausmilzgewebe
Erfolgreiche Detektion in FCA549-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:5000
Durchflusszytometrie (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

WBSee 1 publications below

Produktinformation

10418-1-AP bindet in WB, FC, ELISA CD151 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
In Publikationen genannte ReaktivitätRatte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen CD151 fusion protein Ag0341
Vollständiger Name CD151 molecule (Raph blood group)
Berechnetes Molekulargewicht 28 kDa
Beobachtetes Molekulargewicht 38 kDa
GenBank-ZugangsnummerBC001374
Gene symbol CD151
Gene ID (NCBI) 977
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD151 (also known as TSPAN24) is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Many of these members are involved in the regulation of cell development, activation, growth and motility. CD151 is broadly expressed by a variety of cell types. It is involved in cellular processes including cell adhesion and may regulate integrin trafficking and/or function. CD151 enhances cell motility, invasion and metastasis of cancer cells.

Protokolle

Produktspezifische Protokolle
WB protocol for CD151 antibody 10418-1-APProtokoll herunterladen
FC protocol for CD151 antibody 10418-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
ratWB

FASEB J

Endothelial microparticles induced by cyclic stretch activate Src and modulate cell apoptosis.

Authors - Fei Zhuang
View Article

Int J Mol Med

Tetraspanin CD9 is involved in the migration of retinal microvascular endothelial cells.

Authors - Deissler Heidrun H
View Article