CD16 Monoklonaler Antikörper

CD16 Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / Mouse IgG1, k

Getestete Reaktivität

human

Anwendung

FC

Konjugation

CoraLite® Plus 405 Fluorescent Dye

CloneNo.

3G8

Kat-Nr. : CL405-65090

Synonyme

CD16, CD16A, CD16a antigen, Fc gamma RIII, Fc gamma RIII alpha, Fc gamma RIIIa, FCG3, FCGR3, FCGR3A, FCGRIII, FCR 10, FCRIII, FCRIIIA, IGFR3, IgG Fc receptor III 2



Geprüfte Anwendungen

Erfolgreiche Detektion in FChumane PBMCs

Empfohlene Verdünnung

AnwendungVerdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

CL405-65090 bindet in FC CD16 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / Mouse IgG1, k
Klonalität Monoklonal
Typ Antikörper
Immunogen k.A.
Vollständiger Name Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
Berechnetes Molekulargewicht 254 aa, 29 kDa
GenBank-ZugangsnummerBC017865
Gene symbol FCGR3A
Gene ID (NCBI) 2214
Konjugation CoraLite® Plus 405 Fluorescent Dye
Excitation/Emission maxima wavelengths399 nm / 422 nm
Form Liquid
Reinigungsmethode Affinitätsreinigung
Lagerungspuffer PBS with 0.09% sodium azide and 0.5% BSA.
LagerungsbedingungenStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD16 is a 50-70-kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and the FCGR3B. Clone 3G8 recognizes both the CD16a and CD16b (PMID: 7592758).

Protokolle

Produktspezifische Protokolle
FC protocol for CL Plus 405 CD16 antibody CL405-65090Protokoll herunterladen
Standard-Protokolle
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