CD1e Polyklonaler Antikörper

CD1e Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 22463-1-AP

Synonyme

R2G1, R2, hCD1e, CD1e molecule

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  WB analysis using 22463-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22463-1-AP (CD1e antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 22463-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 22463-1-AP (CD1E antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human plasma using 22463-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 22463-1-AP (CD1E antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 22463-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 22463-1-AP (CD1E antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 22463-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22463-1-AP (CD1E antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 22463-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 22463-1-AP (CD1E antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 22463-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22463-1-AP (CD1E antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, MOLT-4-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

22463-1-AP bindet in WB, ELISA CD1e und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD1e fusion protein Ag18008
• Vollständiger Name: CD1e molecule
• Berechnetes Molekulargewicht: 388 aa, 44 kDa
• Beobachtetes Molekulargewicht: 51 kDa
• GenBank-Zugangsnummer: BC131693
• Gene symbol: CD1E
• Gene ID (NCBI): 913
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD1e molecule (CD1e, also known as R2 and CD1A) is a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin (PMID: 10948205). The discoverers of the CD1 locus originally separated CD1 proteins into group 1 (CD1a, CD1b, and CD1c) and group 2 (CD1d), based on amino acid sequence homology (PMID: 2467814). CD1e is an important modulator of group 1 and group 2 CD1-restricted responses influencing the lipid antigen availability and the generation and persistence of CD1-lipid complexes (PMID: 21844346).