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CD27 Polyklonaler Antikörper

CD27 Polyklonal Antikörper für FC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11121-1-AP

Synonyme

CD27, CD27 antigen, CD27 molecule, CD27L receptor, S152, T cell activation antigen CD27, T14, TNFRSF7, Tp55

Galerie der Validierungsdaten

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  • Western Blot (WB) analysis of Raji cells using CD27 Polyclonal antibody (11121-1-AP)

    WB analysis of Raji using 11121-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 11121-1-AP (CD27 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of Raji cells using CD27 Polyclonal antibody (11121-1-AP)

    FC experiment of Raji using 11121-1-AP

    1X10^6 Raji cells were stained with 0.2ug CD27 antibody (11121-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Geprüfte Anwendungen

Erfolgreiche Detektion in WBRaji-Zellen
Erfolgreiche Detektion in FCRaji-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:1000
Durchflusszytometrie (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

11121-1-AP bindet in WB, FC, ELISA CD27 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen CD27 fusion protein Ag1582
Vollständiger Name CD27 molecule
Berechnetes Molekulargewicht 29 kDa
Beobachtetes Molekulargewicht 45-50 kDa
GenBank-ZugangsnummerBC012160
Gene symbol CD27
Gene ID (NCBI) 939
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD27 (also known as TNFRSF7) is a type I glycoprotein expressed on some B cells and the majority of T cells, and is a member of the tumor necrosis factor (TNF) receptor family. CD27 is required for generation and long-term maintenance of T cell immunity (PMID: 11062504). It is a receptor for CD70 (CD27L). Ligation of CD27 by CD70 induces strong ubiquitination of TRAF and the activation of both canonical and non-canonical NF-kappaB pathways, as well as the JNK pathway (PMID: 19426224). CD27 may also play a role in apoptosis through association with SIVA1.

Protokolle

Produktspezifische Protokolle
WB protocol for CD27 antibody 11121-1-APProtokoll herunterladen
FC protocol for CD27 antibody 11121-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen