CD27 Monoclonal antibody, PBS Only

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  WB analysis using 66308-1-Ig (same clone as 66308-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66308-1-Ig (CD27 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66308-1-PBS in a different storage buffer formulation.

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  WB analysis of pig spleen using 66308-1-Ig (same clone as 66308-1-PBS)

  pig spleen tissue were subjected to SDS PAGE followed by western blot with 66308-1-Ig (CD27 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66308-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66308-1-Ig (same clone as 66308-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66308-1-Ig (CD27 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66308-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66308-1-Ig (same clone as 66308-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66308-1-Ig (CD27 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66308-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66308-1-Ig (same clone as 66308-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66308-1-Ig (CD27 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66308-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 66308-1-Ig (same clone as 66308-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 66308-1-Ig (CD27 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66308-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 66308-1-Ig (same clone as 66308-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CD27 antibody (66308-1-Ig, Clone: 1C1G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66308-1-PBS in a different storage buffer formulation.

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  FC experiment of human PBMCs using 66308-1-Ig (same clone as 66308-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.2 μg CD27 Monoclonal antibody (66308-1-Ig, Clone:1C1G3) or 0.2 μg Mouse IgG1 isotype control (66360-1-Ig, Clone: T1F8D3F10), and CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO.RGAM005). Cells were not fixed. This data was developed using the same antibody clone with 66308-1-PBS in a different storage buffer formulation.

CD27 Monoclonal Antibody for WB, IHC, IF-P, FC, ELISA

Cat No. 66308-1-PBS

Clone No.1C1G3

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IHC, IF-P, FC, ELISA

Synonyms

1C1G3, CD27 antigen, CD27 molecule, CD27L receptor, S152

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Product Information

66308-1-PBS targets CD27 in WB, IHC, IF-P, FC, ELISA applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag24537

  Product name: Recombinant human CD27 protein

  Source: e coli.-derived, PET30a

  Tag: 6*His

  Domain: 1-260 aa of BC012160

  Sequence: MARPHPWWLCVLGTLVGLSATPAPKSCPERHYWAQGKLCCQMCEPGTFLVKDCDQHRKAAQCDPCIPGVSFSPDHHTRPHCESCRHCNSGLLVRNCTITANAECACRNGWQCRDKECTECDPLPNPSLTARSSQALSPHPQPTHLPYVSEMLEARTAGHMQTLADFRQLPARTLSTHWPPQRSLCSSDFIRILVIFSGMFLVFTLAGALFLHQRRKYRSNKGESPVEPAEPCRYSCPREEEGSTIPIQEDYRKPEPACSP

• Full Name: CD27 molecule
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC012160
• Gene Symbol: CD27
• Gene ID (NCBI): 939
• ENSEMBL Gene ID: ENSG00000139193
• RRID: AB_2881690
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P26842
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CD27 (also known as TNFRSF7) is a type I glycoprotein expressed on some B cells and the majority of T cells, and is a member of the tumor necrosis factor (TNF) receptor family. CD27 is required for generation and long-term maintenance of T cell immunity (PMID: 11062504). It is a receptor for CD70 (CD27L). Ligation of CD27 by CD70 induces strong ubiquitination of TRAF and the activation of both canonical and non-canonical NF-kappaB pathways, as well as the JNK pathway (PMID: 19426224). CD27 may also play a role in apoptosis through association with SIVA1.