Tested Applications
| Positive FC detected in | human PBMCs |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 5 ul per 10^6 cells in a 100 µl suspension |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
APC-FcA65570 targets CD3 in FC applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Human infant thymocytes and peripheral blood lymphocytes from a Sézary Syndrome donor Predict reactive species |
| Full Name | CD3e molecule, epsilon (CD3-TCR complex) |
| Calculated Molecular Weight | 207 aa, 23 kDa |
| GenBank Accession Number | BC049847 |
| Gene Symbol | CD3 |
| Gene ID (NCBI) | 916 |
| ENSEMBL Gene ID | ENSG00000198851 |
| RRID | AB_3746418 |
| Conjugate | APC Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 650 nm / 660 nm |
| Excitation Laser | Red Laser (633 nm) |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P07766 |
| Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for APC CD3 antibody APC-FcA65570 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
What customers say
One reviewer (Pedro Andrade) tested this FcZero-rAb APC Anti-Human CD3 antibody on PBMCs by flow cytometry and gave it 5 stars. He reported very visible staining with clear cell population discrimination. A key advantage he highlighted was the elimination of the need for an Fc receptor blocking reagent while still achieving outstanding staining quality. He fully approved and recommended the product.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Pedro (Verified Customer) (06-12-2025) | When I tested the FcZero-rAb APC Anti-Human CD3 (UCHT1) Rabbit IgG Recombinant antibody on PBMCs, I confirmed that the staining was very visible, with the cell population well discriminated. It's an advantage that, with this antibody, I don't need to use FcR Block reagent, and I can't still visualize such an outstanding staining. I approve and recommend the product.
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