CD31 Monoklonaler Antikörper

CD31 Monoklonal Antikörper für FC

Wirt / Isotyp

Ratte / IgG2a, kappa

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

PE Fluorescent Dye

CloneNo.

390

Kat-Nr. : PE-65058

Synonyme

Pecam1, PECAM 1, Pecam, 390

---

• 

  FC experiment of mouse bone marrow cells using PE-65058

  1x10^6 mouse bone marrow cells were surface stained with 0.1 ug PE Anti-Mouse CD31 (PE-65058, Clone: 390) or 0.1 ug PE Rat IgG2a Isotype Control (2A3) (PE-65209, Clone: 2A3). Cells were co-stained with APC Anti-Mouse CD45R (B220) (APC-65139, Clone: RA3-6B2). Cells were not fixed.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Knochenmarkzellen

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

PE-65058 bindet in FC CD31 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2a, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: C3H/HeJ Maus hämatopoetische Vorläuferzelllinie 32D
• Vollständiger Name: platelet/endothelial cell adhesion molecule 1
• GenBank-Zugangsnummer: BC008519
• Gene symbol: CD31
• Gene ID (NCBI): 18613
• Konjugation: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).