CD4 Monoklonaler Antikörper

CD4 Monoklonal Antikörper für FC

Wirt / Isotyp

Ratte / IgG2b, kappa

Getestete Reaktivität

Maus

Anwendung

IF, FC

Konjugation

Unkonjugiert

Publikationen(2)

CloneNo.

GK1.5

Kat-Nr. : 65104-1-Ig

Synonyme

CD4 antigen, GK1.5, L3T4, Ly 4, T-cell differentiation antigen L3T4

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  FC experiment of C57BL/6 mouse splenocytes using 65104-1-Ig

  1x10^6 C57BL/6 mouse splenocytes were surface stained with 0.5 ug Anti-Mouse CD4 (GK1.5) (65104-1-Ig, Clone:GK1.5) or 0.5 ug Rat IgG2b Isotype Control (LTF-2) (65211-1-Ig, Clone: LTF-2) and FITC anti-rat IgG2b Antibody, and 0.5 ug CoraLite® Plus 647 Anti-Mouse CD3 (17A2) (CL647-65077, Clone: 17A2). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: C57BL/6 mouse splenocytes

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below
• FC: See 1 publications below

Produktinformation

65104-1-Ig bindet in IF, FC CD4 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2b, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Maus-CTL-Klon-V4
• Vollständiger Name: CD4 antigen
• GenBank-Zugangsnummer: BC039137
• Gene symbol: Cd4
• Gene ID (NCBI): 12504
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

Publikationen

Species	Application	Title
mouse	FC	Bioact Mater; Photosynthetic microorganisms coupled photodynamic therapy for enhanced antitumor immune effect.; Authors - Haoran Wang
mouse	IF	J Tradit Complement Med; Zhilong Huoxue Tongyu capsule alleviates myocardial fibrosis by improving endothelial cell dysfunction; Authors - Tao Bi