CD44 Rekombinanter Antikörper

CD44 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

F10-44-2

Rezensionen (1)

Kat-Nr. : 65502-1-RR

Synonyme

CD44 antigen, CDW44, CSPG8, ECMR III, ECMR-III

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  FC experiment of human PBMCs using 65502-1-RR

  1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD44 Rabbit Recombinant Antibody (65502-1-RR, Clone:F10-44-2) or Rabbit IgG control Rabbit PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated. Titrations: 0.4, 0.2, 0.1, 0.05ug

• 

  FC experiment of human PBMCs using 65502-1-RR

  1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD44 Rabbit Recombinant Antibody (65502-1-RR, Clone:F10-44-2) or Rabbit IgG control Rabbit PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65502-1-RR

  1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD44 Rabbit Recombinant Antibody (65502-1-PBS, Clone:F10-44-2) (red) and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 or 0.1 ug Anti-Human CD44 Mouse Monoclonal Antibody (Clone:F10-44-2) (65063-1-Ig in green; or from another vendor in pink) and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

65502-1-RR bindet in FC CD44 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Gereinigte T-Zellen aus humanen Lymphknoten
• Vollständiger Name: CD44 molecule (Indian blood group)
• Berechnetes Molekulargewicht: 742 aa, 82 kDa
• GenBank-Zugangsnummer: BC004372
• Gene symbol: CD44
• Gene ID (NCBI): 960
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

CD44 is a type I transmembrane glycoprotein expressed on embryonic stem cells and in various levels on other cell types including connective tissues and bone marrow. CD44 expression is also upregulated in subpopulations of cancer cells and is recognized as a molecular marker for cancer stem cells (PMID: 29747682). It is a cell-surface receptor that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands (PMID: 10694938). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis.

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Chunxu (Verified Customer) (08-08-2025)

I used this anti-CD44 antibody for both immunofluorescence (IF) and flow cytometry on SKOV3 ovarian cancer cells. The antibody performed consistently across experiments, with good signal-to-noise ratio.

• Applications: Immunofluorescence, Flow Cytometry
• Primary Antibody Dilution: 1:250 for IF staining; 0.25ug per 1x10^6 for Flow Cytometry
• Cell Tissue Type: SKOV3

CD44 Antibody Immunofluorescence,Flow Cytometry validation (1:250 for IF staining; 0.25ug per 1x10^6 for Flow Cytometry dilution) in SKOV3 (Cat no:65502-1-RR)