CD44 Monoklonaler Antikörper

CD44 Monoklonal Antikörper für FC

Wirt / Isotyp

Ratte / IgG2b, kappa

Getestete Reaktivität

Maus und mehr (1)

Anwendung

FC

Konjugation

FITC Plus Fluorescent Dye

Publikationen(4)

CloneNo.

IM7

Kat-Nr. : FITC-65117

Synonyme

CD44, CD44 antigen, CDW44, CSPG8, ECMR III, Epican, HCELL, Heparan sulfate proteoglycan, Hermes antigen, HUTCH I, Hyaluronate receptor, IN, LHR, MC56, MDU2, MDU3, MIC4, MUTCH I, PGP 1, PGP I, Pgp1, Phagocytic glycoprotein 1, Phagocytic glycoprotein I

---

• 

  FC experiment of mouse splenocytes using FITC-65117

  1X10^6 mouse splenocytes were surface co-stained with Anti-Mouse CD3 and 0.5 ug FITC Anti-Mouse CD44 (FITC-65117, Clone:IM7) or 0.5 ug Control Antibody. Cells were not fixed.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• FC: See 4 publications below

Produktinformation

FITC-65117 bindet in FC CD44 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Ratte / IgG2b, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Dexamethason-induzierte myeloische Leukämie-M1-Zellen
• Vollständiger Name: CD44 antigen
• GenBank-Zugangsnummer: BC051388
• Gene symbol: Cd44
• Gene ID (NCBI): 12505
• Konjugation: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD44 is a type I transmembrane glycoprotein expressed on embryonic stem cells and in various levels on other cell types including connective tissues and bone marrow. CD44 expression is also upregulated in subpopulations of cancer cells and is recognized as a molecular marker for cancer stem cells (PMID: 29747682). It is a cell-surface receptor that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands (PMID: 10694938). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis.

Publikationen

Species	Application	Title
mouse	FC	Small; The Effect of the Nanoparticle Shape on T Cell Activation.; Authors - Jiwon Oh
mouse	FC	J Control Release; Dual-phase nanoscissors disrupt vasculature-breast cancer stem cell crosstalk for breast cancer treatment; Authors - Yao Qi
human	FC	Cell Mol Life Sci; Phosphocholine inhibits proliferation and reduces stemness of endometrial cancer cells by downregulating mTOR-c-Myc signaling; Authors - Kunxiang Gong
human,mouse	FC	Discov Oncol; Preclinical efficacy of CBR-5884 against epithelial ovarian cancer cells by targeting the serine synthesis pathway; Authors - Kunxiang Gong