CD45 Monoklonaler Antikörper

CD45 Monoklonal Antikörper für FC

Wirt / Isotyp

Ratte / IgG2b, kappa

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

FITC Plus Fluorescent Dye

Publikationen(2)

CloneNo.

30-F11

Kat-Nr. : FITC-65087

Synonyme

Ptprc, 30-F11, EC:3.1.3.48, LCA, L-CA

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  FC experiment of mouse splenocytes using FITC-65087

  1x10^6 mouse splenocytes were surface stained with 0.25 ug FITC Plus Anti-Mouse CD45 (30-F11) (FITC-65087, Clone:30-F11) (red), or FITC Plus Rat IgG2b Isotype Control (LTF-2) (FITC-65211, Clone: LTF-2) (blue). Cells were not fixed.

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  FC experiment of mouse splenocytes using FITC-65087

  1x10^6 mouse splenocytes were surface stained with 0.25 ug FITC Plus Anti-Mouse CD45 (30-F11) (FITC-65087, Clone:30-F11) or FITC Plus Rat IgG2b Isotype Control (LTF-2) (FITC-65211, Clone: LTF-2). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• FC: See 1 publications below

Produktinformation

FITC-65087 bindet in FC CD45 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2b, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Maus-Thymus oder -milz
• Vollständiger Name: protein tyrosine phosphatase, receptor type, C
• GenBank-Zugangsnummer: BC028512
• Gene symbol: CD45
• Gene ID (NCBI): 19264
• Konjugation: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).

Publikationen

Species	Application	Title
mouse	FC	Biomed Pharmacother; Angelica sinensis polysaccharides ameliorated 5-Fluorouracil-induced damage of early B cell progenitors by alleviating oxidative stress of IL-7 producing mesenchymal stem and progenitor cells; Authors - Hanxianzhi Xiao
Mouse		Commun Biol; FOXG1 promotes osteogenesis of bone marrow-derived mesenchymal stem cells by activating autophagy through regulating USP14; Authors - Yiqi Zhang