CoraLite® Plus 750 Anti-Mouse CD45 Rabbit Recombinant Antibody

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  FC experiment of mouse splenocytes using CL750-98035

  1x10^6 mouse splenocytes were surface stained with 0.25 ug CoraLite® Plus 750 Anti-Mouse CD45 Rabbit Recombinant Antibody (CL750-98035, Clone: 240356D1), or CoraLite® Plus 750 Rabbit IgG Isotype Control RecAb (CL750-98136, Clone: 240953C9). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using CL750-98035

  1x10^6 mouse splenocytes were surface stained with 0.25 ug CoraLite® Plus 750 Anti-Mouse CD45 Rabbit Recombinant Antibody (CL750-98035, Clone: 240356D1) (red), or CoraLite® Plus 750 Rabbit IgG Isotype Control RecAb (CL750-98136, Clone: 240953C9) (blue). Cells were not fixed.

CD45 Recombinant Antibody for FC

Cat No. CL750-98035

Clone No.240356D1

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

Ptprc, 240356D1, EC:3.1.3.48, LCA, L-CA

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL750-98035 targets CD45 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CD45 fusion protein Eg0845
• Full Name: protein tyrosine phosphatase, receptor type, C
• Calculated Molecular Weight: 145 kDa
• GenBank Accession Number: NM_001111316.2
• Gene Symbol: CD45
• Gene ID (NCBI): 19264
• RRID: AB_3673797
• Conjugate: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 755 nm / 780 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P06800-4
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).