CD45 Monoklonaler Antikörper

CD45 Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

human

Anwendung

IF, FC

Konjugation

PE Fluorescent Dye

Publikationen(1)

CloneNo.

2D1

Kat-Nr. : PE-65082

Synonyme

2D1, EC:3.1.3.48, GP180, L CA, LCA

---

• 

  FC experiment of human peripheral blood lymphocytes using PE-65082

  1X10^6 human peripheral blood lymphocytes were surface stained with 5 ul PE-Anti-Human CD45 (PE-65082, clone 2D1) (red) or PE-mouse IgG1 isotype control (blue). Cells were not fixed.

• 

  FC experiment of Raji using PE-65082

  1X10^6 Raji cells were surface stained with 5 ul PE Anti-Human CD45 (PE-65082, Clone:2D1) (red), or 5 ul Control Antibody. Cells were not fixed .

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane Lymphozyten aus peripherem Blut, Raji-Zellen

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

PE-65082 bindet in IF, FC CD45 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Humanes PBMC
• Vollständiger Name: protein tyrosine phosphatase, receptor type, C
• GenBank-Zugangsnummer: BC014239
• Gene symbol: CD45
• Gene ID (NCBI): 5788
• Konjugation: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.1% gelatin
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).

Publikationen

Species	Application	Title
human	IF	Exp Ther Med; Detection of PD‑L1 expression and epithelial‑mesenchymal transition of circulating tumor cells in non‑small cell lung cancer; Authors - Jianping Jiang