CD63 Monoklonaler Antikörper

CD63 Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

H5C6

Kat-Nr. : 65295-1-Ig

Synonyme

Lysosome integral membrane protein 1, Lysosomal-associated membrane protein 3, Limp1, H5C6, CD63 antigen

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  FC experiment of human peripheral blood platelets using 65295-1-Ig

  100ul thrombin-activated (red) or unactivated (black dashed) human peripheral blood platelets (diluted 1:2 in PBS) were surface stained with 0.2 ug Anti-Human CD63 (65295-1-Ig, Clone: H5C6) or Mouse IgG1 Isotype Control (65124-1-Ig) (blue) and CoraLite®647-conjugated F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8). Cells were not fixed.

• 

  FC experiment of human peripheral blood platelets using 65295-1-Ig

  100ul thrombin-activated (red) or unactivated (black dashed) human peripheral blood platelets (diluted 1:2 in PBS) were surface stained with 0.2 ug Anti-Human CD63 (65295-1-Ig, Clone: H5C6) or Mouse IgG1 Isotype Control (65124-1-Ig) (blue) and CoraLite®647-conjugated F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: thrombin-activated human peripheral blood platelets

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

65295-1-Ig bindet in FC CD63 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Human splenic adherent cells
• Vollständiger Name: CD63 molecule
• GenBank-Zugangsnummer: BC013017
• Gene symbol: CD63
• Gene ID (NCBI): 967
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD63 is a 30-60 kDa lysosomal membrane protein that belongs to the tetraspanin family. This protein plays many important roles in immuno-physiological functions. It mediates signal transduction events that play a role in the regulation of cell development, activation, growth, and motility. CD63 is expressed on activated platelets, thus it may function as a blood platelet activation marker. CD63 is a lysosomal membrane glycoprotein that is translocated to plasma membrane after platelet activation. The CD63 tetraspanin is highly expressed in the early stages of melanoma and decreases in advanced lesions, suggesting it as a possible suppressor of tumor progression. Deficiency of this protein is associated with Hermansky-Pudlak syndrome.