PE Anti-Human CD63 Rabbit Recombinant Antibody

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  FC experiment of human peripheral blood platelets using PE-98318

  1x10^6 thrombin-treated human peripheral blood platelets were surface stained with 5 ul PE Anti-Human CD63 Rabbit RecAb (PE-98318, Clone: 241964E1) (red) or PE Rabbit IgG Isotype Control RecAb (PE-98136, Clone: 240953C9) (blue). 1x10^6 untreated human peripheral blood platelets were surface stained with 5 ul PE Anti-Human CD63 Rabbit RecAb (PE-98318, Clone: 241964E1) (black, dashed). Cells were not fixed.

CD63 Uni-rAb^TM Recombinant Antibody for FC

Cat No. PE-98318

Clone No.241964E1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

241964E1, CD 63, CD63 antigen, Limp1, Lysosomal-associated membrane protein 3

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Tested Applications

• Positive FC detected in: Thrombin-treated human peripheral blood platelets

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in a 100 µl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

PE-98318 targets CD63 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CD63 fusion protein Eg2064
• Full Name: CD63 molecule
• Calculated Molecular Weight: 26 kDa
• GenBank Accession Number: BC002349
• Gene Symbol: CD63
• Gene ID (NCBI): 967
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P08962
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD63 is a 30-60 kDa lysosomal membrane protein that belongs to the tetraspanin family. This protein plays many important roles in immuno-physiological functions. It mediates signal transduction events that regulate cell development, activation, growth, and motility. CD63 is expressed on activated platelets, thus it may function as a blood platelet activation marker. CD63 is a lysosomal membrane glycoprotein translocated to the plasma membrane after platelet activation. The CD63 tetraspanin is highly expressed in the early stages of melanoma and decreases in advanced lesions, suggesting it is a possible suppressor of tumor progression. Deficiency of this protein is associated with Hermansky-Pudlak syndrome.