CD86 Monoklonaler Antikörper

CD86 Monoklonal Antikörper für IF-P, FC

Wirt / Isotyp

Ratte / IgG2a, kappa

Getestete Reaktivität

Maus

Anwendung

IF-P, FC

Konjugation

FITC Plus Fluorescent Dye

Publikationen(18)

CloneNo.

GL1

Kat-Nr. : FITC-65068

Synonyme

B7, B7 2, B7.2, B70, B7-2

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  IF Staining of mouse spleen using FITC-65068

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using FITC Plus CD86 antibody (FITC-65068, Clone: GL1 ) at dilution of 1:100. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of mouse splenocytes using FITC-65068

  1x10^6 untreated or LPS-treated mouse splenocytes were surface stained with 0.25 ug FITC Plus Anti-Mouse CD86 (GL1) (FITC-65068, Clone: GL1). Cells were not fixed.

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  FC experiment of mouse splenocytes using FITC-65068

  1x10^6 LPS-treated mouse splenocytes were surface stained with 0.25 ug FITC Plus Anti-Mouse CD86 (GL1) (FITC-65068, Clone: GL1) (red) or 0.25 ug FITC Rat IgG2a Isotype Control (2A3) (FITC-65209, Clone: 2A3) (blue). 1x10^6 untreated mouse splenocytes were surface stained with 0.25 ug FITC Plus Anti-Mouse CD86 (GL1) (FITC-65068, Clone: GL1) (black, dashed). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: Mausmilzgewebe
• Erfolgreiche Detektion in FC: LPS treated mouse splenocytes

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 2 publications below
• FC: See 12 publications below

Produktinformation

FITC-65068 bindet in IF-P, FC CD86 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2a, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: LPS-aktivierte CBA/Ca-Mausmilz-B-Zellen
• Vollständiger Name: CD86 antigen
• GenBank-Zugangsnummer: BC013807
• Gene symbol: Cd86
• Gene ID (NCBI): 12524
• Konjugation: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD86 (also known as B7-2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and CTLA-4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with CTLA-4 negatively regulates T-cell activation and diminishes the immune response. (PMID: 7513726; 1847722; 11029388; 27591335)

Publikationen

Species	Application	Title
	FC	Small; [Not Available]; Authors - Baochan Yang
		J Control Release; Programmed antigen capture-harnessed dendritic cells by margination-hitchhiking lung delivery; Authors - Thi My Hue Huynh
mouse	IF	Cell Rep; Mechanotransduction in response to ECM stiffening impairs cGAS immune signaling in tumor cells; Authors - Yingqi Liu
mouse	FC	Carbohydr Polym; Chemical structure and anti-inflammatory activity of a branched polysaccharide isolated from Phellinus baumii.; Authors - Yuqing Sun
		Mol Neurobiol; HPGD: An Intermediate Player in Microglial Polarization and Multiple Sclerosis Regulated by Nr4a1; Authors - Mengyang Sun
mouse	FC	Front Bioeng Biotechnol; Vascular endothelial growth factor attenuates neointimal hyperplasia of decellularized small-diameter vascular grafts by modulating the local inflammatory response; Authors - Xinlong Xie