CD86 Monoklonaler Antikörper

CD86 Monoklonal Antikörper für FC

Wirt / Isotyp

Ratte / IgG2a, kappa

Getestete Reaktivität

Maus

Anwendung

IF, FC

Konjugation

PE Fluorescent Dye

Publikationen(18)

CloneNo.

GL1

Kat-Nr. : PE-65068

Synonyme

B7, B7 2, B7.2, B70, B7-2

---

• 

  FC experiment of mouse splenocytes using PE-65068

  1X10^6 untreated or LPS-treated mouse splenocytes were surface stained with 0.2 ug PE Anti-Mouse CD86 (PE-65068, Clone: GL1). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using PE-65068

  1X10^6 untreated (dashed line) or LPS-treated mouse splenocytes were surface stained with 0.2 ug PE Anti-Mouse CD86 (PE-65068, Clone: GL1) (red), or 0.2 ug Isotype Control (blue). Cells were not fixed.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: LPS treated mouse splenocytes

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 3 publications below
• FC: See 13 publications below

Produktinformation

PE-65068 bindet in IF, FC CD86 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2a, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: LPS-aktivierte CBA/Ca-Mausmilz-B-Zellen
• Vollständiger Name: CD86 antigen
• GenBank-Zugangsnummer: BC013807
• Gene symbol: Cd86
• Gene ID (NCBI): 12524
• Konjugation: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD86 (also known as B7-2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and CTLA-4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with CTLA-4 negatively regulates T-cell activation and diminishes the immune response. (PMID: 7513726; 1847722; 11029388; 27591335)

Publikationen

Species	Application	Title
mouse	FC	Cell Rep; Mechanotransduction in response to ECM stiffening impairs cGAS immune signaling in tumor cells; Authors - Yingqi Liu
mouse	FC	ACS Appl Mater Interfaces; Dual-Regulated Biomimetic Nanocomposites For Promoted Tumor Photodynamic Immunotherapy; Authors - Li Liao
mouse	FC	Int J Nanomedicine; Unexpected Inhibitory Role of Silica Nanoparticles on Lung Cancer Development by Promoting M1 Polarization of Macrophages; Authors - Meng Xiang
	FC	J Integr Neurosci; Avicularin Treatment Ameliorates Ischemic Stroke Damage by Regulating Microglia Polarization and its Exosomes via the NLRP3 Pathway; Authors - Yan Shi
mouse	FC	Naunyn Schmiedebergs Arch Pharmacol; Akkermansia muciniphila relieves inflammatory response in DSS-induced ulcerative colitis in mice through regulating macrophage polarization via SCFAs-SLC52A2/FFAR2 pathway; Authors - Lin Zhang
mouse	IF	Cytokine; Extracellular HSP70 facilitated β-glucan induced trained immunity in macrophages to suppress sepsis via TLR2-NF-κB axis; Authors - Ran Qi