Tested Applications
| Positive FC detected in | LPS treated mouse splenocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.1 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
PE-FcA98025 targets CD86 in FC applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg0802 Product name: Recombinant Mouse CD86 protein (His Tag) Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS Tag: C-6*HIS Domain: 24-245 aa of NM_019388 Sequence: VSVETQAYFNGTAYLPCPFTKAQNISLSELVVFWQDQQKLVLYEHYLGTEKLDSVNAKYLGRTSFDRNNWTLRLHNVQIKDMGSYDCFIQKKPPTGSIILQQTLTELSVIANFSEPEIKLAQNVTGNSGINLTCTSKQGHPKPKKMYFLITNSTNEYGDNMQISQDNVTELFSISNSLSLSFPDGVWHMTVVCVLETESMKISSKPLNFTQEFPSPQTYWKE Predict reactive species |
| Full Name | CD86 antigen |
| Calculated Molecular Weight | 35 kDa |
| GenBank Accession Number | NM_019388 |
| Gene Symbol | Cd86 |
| Gene ID (NCBI) | 12524 |
| RRID | AB_3749698 |
| Conjugate | PE Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 496 nm, 565 nm / 578 nm |
| Excitation Laser | Blue Laser (488 nm), Green Laser (532 nm), Yellow-Green Laser (561 nm) |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P42082-1 |
| Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD86 (also known as B7-2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and CTLA-4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with CTLA-4 negatively regulates T-cell activation and diminishes the immune response. (PMID: 7513726; 1847722; 11029388; 27591335)
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for PE CD86 antibody PE-FcA98025 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
PE-FcA98025 is the FcZero-rAb® Anti-Mouse CD86 Rabbit Recombinant Antibody (clone 230476B8). It has 4 citations published in J Nanobiotechnology, International Journal of Biological Macromolecules, Journal of Biochemical and Molecular Toxicology, and Immunology and Inflammation Disease. The associated research fields include oral drug delivery for ulcerative colitis, immunomodulatory activity of bioactive molecules, glioblastoma immunotherapy, and macrophage efferocytosis in liver abscess.
| Species | Application | Title |
|---|---|---|
J Nanobiotechnology Layer-by-layer engineered outer membrane vesicles enable oral upadacitinib delivery for inflammation control and fibrosis attenuation in ulcerative colitis. | ||
Int J Biol Macromol A low-molecular-weight α-glucan PPS2-A derived from potato starch fermentation by Antarctic Penicillium chrysogenum MS-02: Structural characterization and immunomodulatory activity. | ||
J Biochem Mol Toxicol UCP2 in Glioblastoma Cells Regulates Mitochondrial Metabolic Reprogramming to Mediate Immunophenotypic Repolarization of Tumor-Associated Macrophages and Immunotherapy. | ||
Immun Inflamm Dis METTL3 Alleviates Liver Abscess by Enhancing Macrophage Efferocytosis via m(6)A Methylation of Nrf2 mRNA. |
