CDC25B Polyklonaler Antikörper

CDC25B Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 10644-1-AP

Synonyme

CDC25B, CDC25HU2, M phase inducer phosphatase 2

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Galerie der Validierungsdaten

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  WB analysis of mouse liver using 10644-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10644-1-AP (CDC25B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 10644-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10644-1-AP (CDC25B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10644-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10644-1-AP (CDC25B antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10644-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10644-1-AP (CDC25B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10644-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10644-1-AP (CDC25B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 10644-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10644-1-AP (CDC25B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 10644-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10644-1-AP (CDC25B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 10644-1-AP

  IP Result of anti-CDC25B (IP:10644-1-AP, 4ug; Detection:10644-1-AP 1:300) with mouse liver tissue lysate 4000ug.

• 

  IHC staining of human lymphoma using 10644-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 10644-1-AP (CDC25B antibody) at dilution of 1:100 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, Jurkat-Zellen, Maushirngewebe, Raji-Zellen
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 2 publications below

Produktinformation

10644-1-AP bindet in WB, IP, IHC, IF CDC25B und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CDC25B fusion protein Ag0994
• Vollständiger Name: cell division cycle 25 homolog B (S. pombe)
• Berechnetes Molekulargewicht: 65 kDa
• Beobachtetes Molekulargewicht: 60-70 kDa
• GenBank-Zugangsnummer: BC006395
• Gene symbol: CDC25B
• Gene ID (NCBI): 994
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CDC25B, also named as CDC25HU2, belongs to the MPI phosphatase family. It is a tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. CDC25B directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity. In Jurkat cell, this antibody recognized 2 bands: 60kd(isoform 2, CDC25B2) and 70kd(isoform 4 of CDC25B).

Publikationen

Species	Application	Title
mouse	WB, IF	BMC Dev Biol; Evidence for the requirement of 14-3-3eta (YWHAH) in meiotic spindle assembly during mouse oocyte maturation.; Authors - Santanu De
mouse	IF	BMC Dev Biol; YWHA (14-3-3) protein isoforms and their interactions with CDC25B phosphatase in mouse oogenesis and oocyte maturation.; Authors - Alaa A Eisa