CoraLite®594-conjugated CDC25B Monoclonal antibody

CDC25B Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, rat

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1H3A4

Cat no : CL594-67145

Synonyms

CDC25B, CDC25HU2, M phase inducer phosphatase 2

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Validation Data Gallery

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  IF Staining of Jurkat using CL594-67145

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CoraLite®594 CDC25B antibody (CL594-67145, Clone: 1H3A4 ) at dilution of 1:200.

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  FC experiment of Jurkat using CL594-67145

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human CDC25B (CL594-67145, Clone:1H3A4) (red), or 0.4 ug x. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF detected in: Jurkat cells
• Positive FC detected in: Jurkat cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67145 targets CDC25B in IF, FC (Intra) applications and shows reactivity with Human, Mouse, rat samples.

• Tested Reactivity: Human, Mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: CDC25B fusion protein Ag27193
• Full Name: cell division cycle 25 homolog B (S. pombe)
• Calculated Molecular Weight: 65 kDa
• Observed Molecular Weight: 69 kDa
• GenBank Accession Number: BC006395
• Gene Symbol: CDC25B
• Gene ID (NCBI): 994
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDC25B, also named as CDC25HU2, belongs to the MPI phosphatase family. It is a tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. CDC25B directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.