CDK1 Monoklonaler Antikörper

CDK1 Monoklonal Antikörper für FC (Intra), WB

Wirt / Isotyp

Maus / IgG2a, kappa

Getestete Reaktivität

human, Rind

Anwendung

WB, FC (Intra)

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

POH-1

Kat-Nr. : 65182-1-Ig

Synonyme

CDC2, CDC28A, CDK1, Cell division protein kinase 1, Cyclin dependent kinase 1, DKFZp686L20222, p34 protein kinase

---

Galerie der Validierungsdaten

• 

  WB analysis using 65182-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 65182-1-Ig (CDK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of HeLa using 65182-1-Ig

  1X10^6 HeLa cells were intracellularly stained with 1 ug Anti-Human CDK1 (65182-1-Ig, Clone:POH-1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 1 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (blue). Cells were fixed with 4% PFA and permeabilized with Intracellular Staining Permeabilization Wash Buffer.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HEK-293-Zellen, HeLa-Zellen, K-562-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

65182-1-Ig bindet in WB, FC (Intra) CDK1 und zeigt Reaktivität mit human, Rind

• Getestete Reaktivität: human, Rind
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: In Bakterien exprimiertes humanes Volllängen-Cdk1-Protein
• Vollständiger Name: cell division cycle 2, G1 to S and G2 to M
• Berechnetes Molekulargewicht: 25 kDa
• Beobachtetes Molekulargewicht: 30-34 kDa
• GenBank-Zugangsnummer: BC014563
• Gene symbol: CDC2
• Gene ID (NCBI): 983
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Mit Protein-A-Affinitätschromatographie gereinigt
• Lagerungspuffer: PBS mit 0,1% Natriumazid.
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

CDK1, also named as CDC2, belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and CDC2/CDKX subfamily. CDK1 plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. CDK1 is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. It is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II. Mitotic cyclins stably associate with CDK1 and function as regulatory subunits. CDK1 has 2 isoforms produced by alternative splicing with the molecular mass of 34 kDa and 27 kDa.

Publikationen

Species	Application	Title
human	WB	Cancer Genomics Proteomics; Depletion of DNTTIP2 Induces Cell Cycle Arrest in Pancreatic Cancer Cells; Authors - Masato Yoshizawa