CEP192 Polyklonaler Antikörper

CEP192 Polyklonal Antikörper für IF, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

12

Kat-Nr. : 18832-1-AP

Synonyme

centrosomal protein 192kDa, CEP192, KIAA1569, KIAA1569 protein

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Galerie der Validierungsdaten

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  IP experiment of HeLa cells using 18832-1-AP

  IP result of anti-CEP192 (18832-1-AP for IP and Detection) with HeLa cells.

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  IHC staining of human liver cancer using 18832-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18832-1-AP (CEP192 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 18832-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18832-1-AP (CEP192 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of hTERT-RPE1 using 18832-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using CEP192 antibody (18832-1-AP) at dilution of 1:150 and Cy3-conjugated Affinipure Goat Anti-Rabbit IgG(H+L), SCLT1 antibody (14875-1-AP, Magenta), CoraLite®488 ARL13B antibody (CL488-17711, green).

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  IF Staining of HeLa using 18832-1-AP

  Immunofluorescent analysis of HeLa cells using 18832-1-AP (CEP192 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 18832-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CEP192 antibody (18832-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 18832-1-AP

  Immunofluorescent analysis of HeLa cells using 18832-1-AP (CEP192 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: hTERT-RPE1-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 12 publications below

Produktinformation

18832-1-AP bindet in WB, IP, IHC, IF, ELISA CEP192 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: centrosomal protein 192kDa
• Berechnetes Molekulargewicht: 213 kDa
• Beobachtetes Molekulargewicht: 250-300 kDa
• GenBank-Zugangsnummer: BC144481
• Gene symbol: CEP192
• Gene ID (NCBI): 55125
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CEP192, also named as PP8407 and KIAA1569, interacts with SHBG. It is required for mitotic centrosome and spindle assembly. CEP192 appears to be a major regulator of pericentriolar material (PCM) recruitment, centrosome maturation, and centriole duplication.

Publikationen

Species	Application	Title
mouse	IF	Science; Mechanism of spindle pole organization and instability in human oocytes.; Authors - Chun So
mouse	IF	Science; A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes.; Authors - Chun So
mouse	IF	EMBO Rep; ENKD1 promotes CP110 removal through competing with CEP97 to initiate ciliogenesis.; Authors - Ting Song
human	WB,IF	PLoS Biol; The ciliopathy protein CCDC66 controls mitotic progression and cytokinesis by promoting microtubule nucleation and organization.; Authors - Umut Batman
mouse	IF	PLoS Genet; Aurora kinase A is essential for meiosis in mouse oocytes.; Authors - Cecilia S Blengini
human	IF	RNA; Human Nuclear RNAi-Defective 2 (NRDE2) is an essential RNA splicing factor.; Authors - Alan L Jiao