CRH/CRF Polyklonaler Antikörper

CRH/CRF Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (3)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

25

Rezensionen (2)

Kat-Nr. : 10944-1-AP

Synonyme

Corticoliberin, CRF, CRH

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Galerie der Validierungsdaten

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  WB analysis of human brain using 10944-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10944-1-AP (CRH/CRF antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 10944-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10944-1-AP (CRH/CRF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 10944-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10944-1-AP (CRH/CRF antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 10944-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10944-1-AP (CRH/CRF antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 10944-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10944-1-AP (CRH/CRF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 10944-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10944-1-AP (CRH/CRF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 10944-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CRH/CRF antibody (10944-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 10944-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CRH/CRF antibody (10944-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 13 publications below
• IHC: See 11 publications below
• IF: See 11 publications below

Produktinformation

10944-1-AP bindet in WB, IHC, IF, ELISA CRH/CRF und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte, Zebrafisch, Präriewühlmaus (Microtus ochrogaster)
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CRH/CRF fusion protein Ag1371
• Vollständiger Name: CRH/CRF
• Berechnetes Molekulargewicht: 21 kDa
• Beobachtetes Molekulargewicht: 35-38 kDa
• GenBank-Zugangsnummer: BC011031
• Gene symbol: CRH
• Gene ID (NCBI): 1392
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CRH, also called CRF or corticoliberin, is a peptide hormone and neurotransmitter involved in the stress response. Marked reduction in this protein has been observed in association with Alzheimer disease. In addition to production in the hypothalamus, this protein is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed in the placenta. In the placenta it is a marker that determines the length of gestation and the timing of parturition and delivery.

Publikationen

Species	Application	Title
Microtus Ochrogaster	WB	Neurobiol Stress; Social isolation alters behavior, the gut-immune-brain axis, and neurochemical circuits in male and female prairie voles.; Authors - Meghan Donovan
human	IHC,IF	Elife; Single-cell transcriptome analysis identifies a unique tumor cell type producing multiple hormones in ectopic ACTH and CRH secreting pheochromocytoma.; Authors - Xuebin Zhang
human	IHC,IF	Brain Behav Immun; Defects in NLRP6, autophagy and goblet cell homeostasis are associated with reduced duodenal CRH receptor 2 expression in patients with functional dyspepsia.; Authors - Jessica K Bruce
human	IF	Int J Mol Sci; Stress and Nasal Allergy: Corticotropin-Releasing Hormone Stimulates Mast Cell Degranulation and Proliferation in Human Nasal Mucosa.; Authors - Mika Yamanaka-Takaichi
mouse	IHC	Development; PLXNA1 and PLXNA3 cooperate to pattern the nasal axons that guide gonadotropin-releasing hormone neurons.; Authors - Roberto Oleari
zebrafish	WB, IF	Neuropharmacology; PMID: 25842247; Authors - Fengjiao Chen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

roberto (Verified Customer) (06-07-2019)

High specific signal

• Applications: Immunohistochemistry, Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: postnatal mouse brain

CRH/CRF Antibody Immunohistochemistry,Immunofluorescence, validation (1:100 dilution) in postnatal mouse brain (Cat no:10944-1-AP)

FH

Yan (Verified Customer) (10-31-2017)

• Applications: Western Blot
• Primary Antibody Dilution: 1:10000
• Cell Tissue Type: human postmortem brain

CRH/CRF Antibody Western Blot validation (1:10000 dilution) in human postmortem brain (Cat no:10944-1-AP)

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