CX3CR1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CX3CR1 Polyklonal Antikörper für FC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IP, IHC, IF, FC, CoIP, Cell treatment, ELISA

Konjugation

Unkonjugiert

Publikationen

30

Rezensionen (2)

Kat-Nr. : 13885-1-AP

Synonyme

Beta chemokine receptor like 1, C X3 C CKR 1, CCRL1, CMK BRL 1, CMK BRL1, CMKBRL1, CMKDR1, CX3C chemokine receptor 1, CX3CR1, Fractalkine receptor, G protein coupled receptor 13, GPR13, GPRV28, V28

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 13885-1-AP

  WB result of CX3CR1 antibody (13885-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CX3CR1 transfected HEK-293 cells.

• 

  WB analysis using 13885-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13885-1-AP (CX3CR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 13885-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 13885-1-AP (CX3CR1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 13885-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 13885-1-AP (CX3CR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 13885-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 13885-1-AP (CX3CR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 13885-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13885-1-AP (CX3CR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of SH-SY5Y using 13885-1-AP

  IP Result of anti-CX3CR1 (IP:13885-1-AP, 4ug; Detection:13885-1-AP 1:1000) with SH-SY5Y cells lysate 1600ug.

• 

  FC experiment of K-562 using 13885-1-AP

  1X10^6 K-562 cells were stained with 0.2ug CX3CR1 antibody (13885-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, HL-60-Zellen, K-562-Zellen, SH-SY5Y-Zellen, U-937-Zellen
• Erfolgreiche IP: SH-SY5Y-Zellen
• Erfolgreiche Detektion in FC: K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 22 publications below
• IHC: See 11 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• FC: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

13885-1-AP bindet in WB, IP, IHC, IF, FC, CoIP, Cell treatment, ELISA CX3CR1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CX3CR1 fusion protein Ag4149
• Vollständiger Name: chemokine (C-X3-C motif) receptor 1
• Berechnetes Molekulargewicht: 40 kDa
• Beobachtetes Molekulargewicht: 44-47 kDa
• GenBank-Zugangsnummer: BC028078
• Gene symbol: CX3CR1
• Gene ID (NCBI): 1524
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CX3CR1, also known as GPR13, V28 and fractalkine receptor, belongs to the 7-transmembrane G protein-coupled receptor (GPCR) family. It is expressed on microglia, astrocytes, NK cells, monocytes/macrophages, and a subpopulation of T cells. CX3CR1 is the receptor for fractalkine (CX3CL1) and mediates both its adhesive and migratory functions. It also acts as coreceptor with CD4 for HIV-1 virus envelope protein (in vitro), and some variations in the gene of CX3CR1 lead to increased susceptibility to HIV-1 infection and rapid progression to AIDS. Defects in CX3CR1 are a cause of susceptibility to age-related macular degeneration type 12 (ARMD12).

Publikationen

Species	Application	Title
mouse	IP	Brain; Reactive microglia enhance the transmission of exosomal alpha-synuclein via toll-like receptor 2.; Authors - Yun Xia
human	WB,IF	Tissue Cell; Analysis of Aβ-induced neurotoxicity and microglial responses in simple two- and three-dimensional human iPSC-derived cortical culture systems; Authors - Mayuko Takata
mouse	WB	J Control Release; Chemoattractants driven and microglia based biomimetic nanoparticle treating TMZ-resistant glioblastoma multiforme.; Authors - Sai Qiao
mouse	WB	Nanoscale; Calming egress of inflammatory monocytes and related septic shock by therapeutic CCR2 silencing using macrophage-derived extracellular vesicles.; Authors - Liang Ding
human	WB	Mol Oncol; Platelets are recruited to hepatocellular carcinoma tissues in a CX3CL1-CX3CR1 dependent manner and induce tumour cell apoptosis.; Authors - Shuo Miao
mouse	WB,Cell treatment	J Nanobiotechnology; Biomimetic GBM-targeted drug delivery system boosting ferroptosis for immunotherapy of orthotopic drug-resistant GBM.; Authors - Bao Liu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Reyes (Verified Customer) (03-01-2024)

Cx3cr1 antibody (in red) tried on human paraffin brain sections, apparently vesicular while marking my microglia.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Brain Cortex

CX3CR1 Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:13885-1-AP)

FH

Ryan (Verified Customer) (06-15-2018)

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: zebrafish retina

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