ERK1/2 Polyklonaler Antikörper

ERK1/2 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (3)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

382

Rezensionen (2)

Kat-Nr. : 16443-1-AP

Synonyme

ERK, ERK 2, ERK1/2, ERK2, ERT1, MAP kinase 1, MAP kinase 2, MAP kinase isoform p42, MAPK 1, MAPK 2, MAPK1, MAPK2, p40, p41, p41mapk, p42 MAPK, P42MAPK, PRKM1, PRKM2

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Galerie der Validierungsdaten

• 

  WB analysis of NIH/3T3 using 16443-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16443-1-AP (ERK1/2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 16443-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 16443-1-AP (ERK1/2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 16443-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 16443-1-AP (ERK1/2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 16443-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16443-1-AP (ERK1/2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 16443-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16443-1-AP (ERK1/2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16443-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16443-1-AP (ERK1/2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 16443-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16443-1-AP (ERK1/2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 16443-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16443-1-AP (ERK1/2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 16443-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16443-1-AP (ERK1/2 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human kidney using 16443-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 16443-1-AP (ERK1/2 Antibody) at dilution of 1:50.

• 

  IHC staining of human kidney using 16443-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 16443-1-AP (ERK1/2 Antibody) at dilution of 1:50.

• 

  IF Staining of HeLa using 16443-1-AP

  Immunofluorescent analysis of HeLa cells using 16443-1-AP (ERK1/2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen, A431-Zellen, HEK-293-Zellen, HeLa-Zellen, Maushirngewebe, Mausnierengewebe, PC-3-Zellen, Rattenhirngewebe, Rattennierengewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 373 publications below
• IHC: See 5 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Produktinformation

16443-1-AP bindet in WB, IP, IHC, IF, ELISA ERK1/2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Huhn, Maus, Ratte, Chinesische Wollhandkrabbe (Eriocheir sinensis)
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ERK1/2 fusion protein Ag9772
• Vollständiger Name: mitogen-activated protein kinase 1
• Berechnetes Molekulargewicht: 360 aa, 41 kDa
• Beobachtetes Molekulargewicht: 38-44 kDa
• GenBank-Zugangsnummer: BC017832
• Gene symbol: MAPK1
• Gene ID (NCBI): 5594
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-44 kDa.

Publikationen

Species	Application	Title
human	WB	J Immunother Cancer; TSH-TSHR axis promotes tumor immune evasion.; Authors - Zhenghao Wu
rat	WB	Adv Sci (Weinh); An Important Function of Petrosiol E in Inducing the Differentiation of Neuronal Progenitors and in Protecting Them against Oxidative Stress.; Authors - Jing Liu
human	WB	J Hazard Mater; ZnO NPs delay the recovery of psoriasis-like skin lesions through promoting nuclear translocation of p-NFκB p65 and cysteine deficiency in keratinocytes.; Authors - Xuan Lai
human	WB,IP	Cell Death Dis; 4EBP1 senses extracellular glucose deprivation and initiates cell death signaling in lung cancer; Authors - Yanan Wang
human	WB	Mol Ther; Exosomal miR-1246 from glioma patient body fluids drives the differentiation and activation of myeloid-derived suppressor cells.; Authors - Wei Qiu
human	WB	Clin Cancer Res; Inhibition of Bcl-2 Synergistically Enhances the Antileukemic Activity of Midostaurin and Gilteritinib in Preclinical Models of FLT3-mutated Acute Myeloid Leukemia.; Authors - Jun Ma

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Macarena Lucia (Verified Customer) (10-17-2022)

good results on human lysates

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000

FH

Tom (Verified Customer) (09-29-2020)

10ug of HEK293T total cell lysate in SDS loading buffer was loaded and ran on a 4-20% gradient gel. Blocking was performed in 5% BSA in TBS for 1 hour then membrane incubated at 4 degrees overnight with anti-ERK 1/2 antibody (1:1000) in block. A goat anti-rabbit HRP secondary antibody (1:10,000) was used.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

ERK1/2 Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:16443-1-AP)

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