ERO1LB Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ERO1LB Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 11261-2-AP

Synonyme

ERO1 L beta, ERO1 like beta (S. cerevisiae), ERO1 like protein beta, ERO1B, ERO1LB, Oxidoreductin 1 L beta

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 11261-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11261-2-AP (ERO1LB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 11261-2-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 11261-2-AP (ERO1LB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11261-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11261-2-AP (ERO1LB antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 11261-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11261-2-AP (ERO1LB antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human small intestine using 11261-2-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 11261-2-AP (ERO1LB antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human small intestine using 11261-2-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 11261-2-AP (ERO1LB antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human oesophagus using 11261-2-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus using 11261-2-AP (ERO1LB antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human oesophagus using 11261-2-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus using 11261-2-AP (ERO1LB antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 11261-2-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 11261-2-AP (ERO1LB antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe, humanes Dünndarmgewebe, humanes Ösophagusgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 6 publications below

Produktinformation

11261-2-AP bindet in WB, IHC, IF, ELISA ERO1LB und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ERO1LB fusion protein Ag1776
• Vollständiger Name: ERO1-like beta (S. cerevisiae)
• Berechnetes Molekulargewicht: 54 kDa
• Beobachtetes Molekulargewicht: 40~50 kDa
• GenBank-Zugangsnummer: BC032823
• Gene symbol: ERO1LB
• Gene ID (NCBI): 56605
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Endoplasmic reticulum oxidoreductases (Eros) are essential for the formation of disulfide bonds. Mammals express two related Ero proteins, Ero1L alpha and Ero1L beta. The two homologous proteins can form homodimers and mixed heterodimers. ERO1-Lα and -β are highly expressed in organs with a high demand for secretion, consistent with their roles in oxidative folding of ER proteins.

Publikationen

Species	Application	Title
mouse	WB	Hum Mol Genet; Curcumin Facilitates a Transitory Cellular Stress Response in Trembler-J Mice.; Authors - Okamoto Yuji Y
mouse	WB	Endocrinology; PMID: 21540283; Authors - Khoo Cynthia C ; KD Validated
human	WB	J Biol Chem; Action of protein disulfide isomerase on proinsulin exit from endoplasmic reticulum of pancreatic β-cells.; Authors - Rajpal Gautam G
human	WB	Sci Rep; Hypoxia-inducible ERO1α promotes cancer progression through modulation of integrin-β1 modification and signalling in HCT116 colorectal cancer cells.; Authors - Norio Takei ; KO Validated
mouse	WB	J Toxicol; Oxidative Stress and the ER Stress Response in a Murine Model for Early-Stage Alcoholic Liver Disease.; Authors - Galligan James J JJ
human	WB	ACS Bio Med Chem Au; Identification of Natural Product Sulfuretin Derivatives as Inhibitors for the Endoplasmic Reticulum Redox Protein ERO1α; Authors - Brennan D Johnson ; KO Validated