Ephrin B2 Recombinant monoclonal antibody

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• Ephrin B2 Recombinant monoclonal antibody (87995-1-RR)

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  WB analysis using 87995-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87995-1-RR (EFNB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Ephrin B2 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87995-1-RR

Clone No.253599B5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

EFNB2, EPLG5, EPH-related receptor tyrosine kinase ligand 5, Ephrin-B2, Htk L

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Tested Applications

• Positive WB detected in: HeLa cells, HT-29 cells, NIH/3T3 cells, mouse spleen tissue, mouse placenta tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87995-1-RR targets Ephrin B2 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag24865

  Product name: Recombinant human EFNB2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 168-235 aa of BC074856

  Sequence: ASSAGSTRNKDPTRRPELEAGTNGRSSTTSPFVKPNPGSSTDGNSAGHSGNNILGSEVALFAGIASGC

• Full Name: ephrin-B2
• Calculated Molecular Weight: 333 aa, 37 kDa
• Observed Molecular Weight: 47-50 kDa
• GenBank Accession Number: BC074856
• Gene Symbol: EFNB2
• Gene ID (NCBI): 1948
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P52799
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ephrin B2 (EFNB2) is a transmembrane ligand for the Ephrin B class (EPHB) of receptor tyrosine kinases. EPHB receptor(EPHB1-EPHB4, EPH6) are located at the member of one cell and activated by binding EFNB protein located on another cell, resulting in a bidirectional Ephrin-EphR signaling (PMID: 26790531). Ephrin-B are involved in vasculogenesis, neural development and synaptic plasticity, and in pathological processes such as cancer or neurodegeneration (PMID:29508392). The calculated molecular weight of EFNB2 is 37 kDa, and there is also detected about 50 kDa band in the article (PMID: 27650867, PMID: 23492730).