Tested Applications
| Positive WB detected in | human plasma tissue, human blood tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66722-1-Ig targets Factor VIII in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag15772 Product name: Recombinant human F8 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 2144-2351 aa of BC022513 Sequence: VFFGNVDSSGIKHNIFNPPIIARYIRLHPTHYSIRSTLRMELMGCDLNSCSMPLGMESKAISDAQITASSYFTNMFATWSPSKARLHLQGRSNAWRPQVNNPKEWLQVDFQKTMKVTGVTTQGVKSLLTSMYVKEFLISSSQDGHQWTLFFQNGKVKVFQGNQDSFTPVVNSLDPPLLTRYLRIHPQSWVHQIALRMEVLGCEAQDLY Predict reactive species |
| Full Name | coagulation factor VIII, procoagulant component |
| Calculated Molecular Weight | 2351 aa, 267 kDa |
| Observed Molecular Weight | 70 kDa |
| GenBank Accession Number | BC022513 |
| Gene Symbol | F8 |
| Gene ID (NCBI) | 2157 |
| RRID | AB_2882073 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P00451 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
F8, also named as FVIII, AHF, and F8C, belongs to the multicopper oxidase family. F8, along with calcium and phospholipid, acts as a cofactor for factor IXa when it converts factor X to the activated form, factor Xa. FVIII is translated into a single-chain polypeptide (SCh) with the A1-A2-B-A3-C1-C2 domain, which can undergo glycosylation, sulfonation, and variable cleavage. The FVIII molecule is a heterodimer composed of a heavy chain (HCh, A1-A2-B domain, 90-210 kDa) and a light chain (LCh, A3-C1-C2 domain, 70-80 kDa)(PMID: 28109042; 18650448; 29292164). This antibody can recognize the light chain.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Factor VIII antibody 66722-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Factor VIII Monoclonal antibody (1C11E11), catalog number 66722-1-Ig, has a total of 2 citations. These appear in Vet Res (2024), studying TRIM28 regulation of the coagulation cascade in African swine fever virus infection in pigs, and J Thorac Dis (2025), examining circRNA differential expression in human pulmonary microvascular endothelial cells during acute respiratory distress syndrome. The associated research fields span veterinary virology/coagulation biology and human pulmonary/respiratory pathology.
| Species | Application | Title |
|---|---|---|
Vet Res TRIM28 regulates the coagulation cascade inhibited by p72 of African swine fever virus
| ||
J Thorac Dis Transcriptome sequencing showed the differential expression of circRNAs in human pulmonary microvascular endothelial cells in acute respiratory distress syndrome |
