FOXP3 Polyklonaler Antikörper

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• KD/KO Validated

FOXP3 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 22173-1-AP

Synonyme

AIID, DIETER, forkhead box P3, Forkhead box protein P3, FOXP3, IPEX, JM2, PIDX, Scurfin, XPID

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  WB analysis of HEK-293 using 22173-1-AP

  WB result of FOXP3 antibody (22173-1-AP, 1:500) with si-Control and si-FOXP3 transfected HEK293 cells.

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  WB analysis of HEK-293 using 22173-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22173-1-AP (FOXP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW264.7 using 22173-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 22173-1-AP (FOXP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 22173-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22173-1-AP (FOXP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 22173-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22173-1-AP (FOXP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW264.7 using 22173-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 22173-1-AP (FOXP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW264.7 using 22173-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 22173-1-AP (FOXP3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 22173-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22173-1-AP (FOXP3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 22173-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 22173-1-AP (FOXP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 22173-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22173-1-AP (FOXP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 22173-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22173-1-AP (FOXP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HEK-293

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

22173-1-AP bindet in WB, IHC, ELISA FOXP3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FOXP3 fusion protein Ag17827
• Vollständiger Name: forkhead box P3
• Berechnetes Molekulargewicht: 431 aa, 47 kDa
• Beobachtetes Molekulargewicht: 47-50 kDa
• GenBank-Zugangsnummer: BC113401
• Gene symbol: FOXP3
• Gene ID (NCBI): 50943
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FOXP3, also named as IPEX, JM2 and Scurfin, is a transcription factor. FOXP3 plays a critical role in the control of immune response. Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) which also known as X-linked autoimmunity-immunodeficiency syndrome. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human FOXP3.

Publikationen

Species	Application	Title
mouse	WB	Exp Ther Med; Poly(ADP-ribose) polymerase-1 inhibitor ameliorates dextran sulfate sodium-induced colitis in mice by regulating the balance of Th17/Treg cells and inhibiting the NF-κB signaling pathway.; Authors - Shuai Peng
human	IHC	Oral Dis; Influence of PD-1/PD-L1 on immune micro-environment in oral leukoplakia and oral squamous cell carcinomas; Authors - Qiong Wang