GSK3B Polyklonaler Antikörper

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• KD/KO Validated

GSK3B Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 15113-1-AP

Synonyme

GSK 3 beta, GSK3 beta, GSK3A B, GSK3B, GSK3B 216P, gsk3beta

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Galerie der Validierungsdaten

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  WB analysis of A549 using 15113-1-AP

  WB result of GSK3B antibody (15113-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSK3B transfected A549 cells.

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  WB analysis of mouse ovary using 15113-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 15113-1-AP (GSK3B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15113-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15113-1-AP (GSK3B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 15113-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15113-1-AP (GSK3B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 15113-1-AP

  IP Result of anti-GSK3B (IP:15113-1-AP, 4ug; Detection:15113-1-AP 1:500) with mouse heart tissue lysate 4000ug.

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  IHC staining of human testis using 15113-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 15113-1-AP (GSK3B Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 15113-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 15113-1-AP (GSK3B Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Eierstockgewebe, A549-Zellen, HEK-293-Zellen, HeLa-Zellen
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 9 publications below

Produktinformation

15113-1-AP bindet in WB, IP, IHC, ELISA GSK3B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GSK3B fusion protein Ag7101
• Vollständiger Name: glycogen synthase kinase 3 beta
• Berechnetes Molekulargewicht: 433 aa, 48 kDa
• Beobachtetes Molekulargewicht: 55-60 kDa
• GenBank-Zugangsnummer: BC000251
• Gene symbol: GSK3B
• Gene ID (NCBI): 2932
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase .GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.In skeletal muscle, it contributes to INS regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Researches showed that the crystal structure of human GSK3B, expressed in insect cells, at 2.8-angstrom resolution .This antibody recognize the 2 isoforms of GSK3B.

Publikationen

Species	Application	Title
human	WB	Front Oncol; The Role of miR-640: A Potential Suppressor in Breast Cancer via Wnt7b/β-catenin Signaling Pathway.; Authors - Chun Tang
human	WB	J Ethnopharmacol; Entada phaseoloides extract suppresses hepatic gluconeogenesis via activation of the AMPK signaling pathway.; Authors - Tao Zheng
mouse	WB	Biochem Biophys Res Commun; Perilipin 5 ameliorates high-glucose-induced podocyte injury via Akt/GSK-3β/Nrf2-mediated suppression of apoptosis, oxidative stress, and inflammation.; Authors - Jie Feng
human	WB	Biochem Biophys Res Commun; NOL4L, a novel nuclear protein, promotes cell proliferation and metastasis by enhancing the PI3K/AKT pathway in ovarian cancer.; Authors - Feikai Lin
human	WB	Microvasc Res; Luteolin alleviates ischemia/reperfusion injury-induced no-reflow by regulating Wnt/β-catenin signaling in rats.; Authors - Xichun Qin
human	WB	Cell Biol Int; Identification and analysis of glycogen synthase kinase 3 beta1 interactome.; Authors - Gao Xuejuan X