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IFNAR1 Polyklonaler Antikörper
IFNAR1 Polyklonal Antikörper für FC, WB, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus
Anwendung
WB, IF, FC, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 13083-1-AP
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | Jurkat-Zellen, Mauslebergewebe |
| Erfolgreiche Detektion in FC | K-562-Zellen |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Durchflusszytometrie (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| KD/KO | See 1 publications below |
| WB | See 7 publications below |
| IF | See 1 publications below |
Produktinformation
13083-1-AP bindet in WB, IF, FC, ELISA IFNAR1 und zeigt Reaktivität mit human, Maus
| Getestete Reaktivität | human, Maus |
| In Publikationen genannte Reaktivität | human |
| Wirt / Isotyp | Kaninchen / IgG |
| Klonalität | Polyklonal |
| Typ | Antikörper |
| Immunogen | IFNAR1 fusion protein Ag3739 |
| Vollständiger Name | interferon (alpha, beta and omega) receptor 1 |
| Berechnetes Molekulargewicht | 557 aa, 64 kDa |
| Beobachtetes Molekulargewicht | 64 kDa |
| GenBank-Zugangsnummer | BC021825 |
| Gene symbol | IFNAR1 |
| Gene ID (NCBI) | 3454 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Antigen-Affinitätsreinigung |
| Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Interferon alpha and beta receptor subunit 1 (IFNAR1) is a type I membrane protein that associates with IFNAR2 to form the receptor for type I interferons, including interferons-alpha, -beta, and -lambda. Binding and activation of the receptor stimulates Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2, and then increase transcription of the IFN-induced genes whose products exert antiviral, immunomodulatory, and antiproliferative effects.
Protokolle
| Produktspezifische Protokolle | |
|---|---|
| WB protocol for IFNAR1 antibody 13083-1-AP | Protokoll herunterladen |
| FC protocol for IFNAR1 antibody 13083-1-AP | Protokoll herunterladen |
| Standard-Protokolle | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
EMBO Rep Degradation of WTAP blocks antiviral responses by reducing the m 6 A levels of IRF3 and IFNAR1 mRNA | ||
Elife The oncoprotein BCL6 enables solid tumor cells to evade genotoxic stress.
| ||
Cell Rep TDP-43 prevents endogenous RNAs from triggering a lethal RIG-I-dependent interferon response. | ||
J Nat Med Baicalin promotes antiviral IFNs production and alleviates type I IFN-induced neutrophil inflammation |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Christine (Verified Customer) (07-12-2022) | Both cell lines are human ones, only the RPE1 cells express the IFNAR1 receptor. IFNAR1 is described in the literature as heavily glycosylated and hence its molecular weight can vary from 64-135 kDa depending on its glycosylation level. We generally observed in RPE1 cells fully matured 110-130 kDa major band on western blot, which is not observed at all here with the 13083-1-AP antibody. The 72 kDa bands seems to be present only in RPE1 and not in the IFNAR1 null Huh7 cells, indicating it could indeed be immature non glycosylated forms of IFNAR1. (We also observed these minor bands on RPE1 cells with another IFNAR1 antibody , but at a much lower intensity than the 110 kDa one)
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